We narrowed to 19,741 results for: Cre

1. pCMV_EE-NG-nCas9

   Plasmid

   #163542

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   EE-NG-nCas9
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Dec. 21, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. Unc5c.a-Fc-His

   Plasmid

   #72180

   Purpose

   Expresses the extracellular region of the Unc5C, isoform a protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Unc5c.a (Unc5c Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 26, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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3. Ncam1_0.0.0-AP-His

   Plasmid

   #71960

   Purpose

   Expresses the extracellular region of the NCAM1, isoform 0.0.0 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Ncam1_0.0.0 (Ncam1 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 25, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pJS699

   Plasmid

   #168779

   Purpose

   Encodes the SARS-CoV-2 Spike Receptor Binding Domain (RBD) and can be combined with pJS697 through homologous recombination

   Depositor

   Timothy Whitehead

   Article

   Francino-Urdaniz et al Cell Rep. 2021 Aug 31;36(9):109627. doi:

   Insert

   Spike RBD (S betacoronavirus)
   

   Tags

   Cmyc

   Mutation

   N343Q

   Promoter

   GAL1

   Available Since

   Aug. 31, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. SUMO3_pcDNA6.2/EmGFP-Bsd

   Plasmid

   #176949

   Purpose

   Mammalian expression vector encoding SUMO3 and EmGFP-Bsd

   Depositor

   Roger Reeves

   Article

   Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

   Insert

   SUMO3 (SUMO3 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. EGFP-uTEV1-MAVS-mTA4

   Plasmid

   #171002

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI] C-terminal tmd targeting information (Mitochondria and ER insertion)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI]
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI]

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pcDNA5FRT/TO-YOD1-Strep-HA

   Plasmid

   #113483

   Purpose

   Expression of human YOD1 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   YOD1 (YOD1 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA-Cx43-Myc

   Plasmid

   #60693

   Purpose

   mammalian expression of Myc-tagged rat Cx43

   Depositor

   Parmender Mehta

   Article

   Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

   Insert

   Cx43 (Gja1 Rat)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 6, 2015

   Availability

   Academic Institutions and Nonprofits only

9. Sema3a(L, del13)-AP-His

   Plasmid

   #72011

   Purpose

   Expresses the Sema3A protein (truncated at cleavage site P3; ie, long and missing exon 13), C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Sema3a (Sema3a Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 22, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. KO101: pMAGIC (R4-R3) NLS-x dCas9(3.7)-NLS-Dnmt3a3L

    Plasmid

    #121833

    Purpose

    pMAGIC R4-R3 entry plasmid, contains 2x NLS x-dCas9(3.7) fused to the Dnmt3a-3L DNA methyltransferase for 3 or 4-component MultiSite Gateway Pro assembly.

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    x-dCas9(3.7)/Dnmt3a-3L (open)
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Tags

    NLS

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. p3E-CDC42

    Plasmid

    #109583

    Purpose

    Multisite gateway vector for 3' tagging with CDC42

    Depositor

    Rob Parton

    Article

    Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

    Insert

    CDC42 (cdc42 Zebrafish)
    

    Use

    Zebrafish plasmids

    Available Since

    Feb. 27, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. HP-Cas9hGem-Ade2-LEU2-LINEAR

    Plasmid

    #174839

    Purpose

    pRS414 backbone containing LINEAR fragment targeting ADE2 in H. polymorpha

    Depositor

    Zengyi Shao

    Article

    Ploessl et al Nat Chem Biol. 2021 Oct 28. pii: 10.1038

    Insert

    HH-gRNA-HDV targetting ADE2 in Ogataea (para)polymorpha
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Bacterial and Yeast

    Promoter

    TDH3p

    Available Since

    Jan. 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLNCX1 myr Akt1 delta4-129

    Plasmid

    #15989

    Depositor

    Morris Birnbaum

    Article

    Tuttle et al Nat Med. 2001 Oct . 7(10):1133-7.

    Insert

    Akt1 (AKT1 Human)
    

    Use

    Retroviral

    Tags

    HA and Myr

    Expression

    Mammalian

    Mutation

    Delta 4-129

    Available Since

    Oct. 26, 2007

    Availability

    Academic Institutions and Nonprofits only

14. pGMC00034 (aka pMC0235)

    Plasmid

    #210751

    Purpose

    Non-targeting guide RNA

    Depositor

    Raj Chari

    Article

    Ohara et al Cell Rep. 2023 Nov 18;42(12):113434. doi

    Insert

    Non-targeting guide RNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Jan. 19, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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15. pKLV2-U6gRNA5(Nprl2-g1)-PGKpuroBFP-W

    Plasmid

    #105037

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pKLV2-U6gRNA5(Nprl2-g2)-PGKpuroBFP-W

    Plasmid

    #105038

    Purpose

    Lentiviral gRNA plasmid targeting mouse Nprl2 , co-expression of TagBFP

    Depositor

    Kosuke Yusa

    Article

    Li et al Cell Rep. 2018 Jul 10;24(2):489-502. doi

    Insert

    Nprl2 (Nprl2 Mouse)
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Sept. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. CNNM3-CBS-YPet

    Plasmid

    #149686

    Purpose

    For the FRET-based binding assay between human CNNM3 CBS domain and PRL2.

    Depositor

    Motoyuki Hattori

    Article

    Cai et al Sci Rep. 2020 Jul 30;10(1):12879. doi: 1

    Insert

    CNNM3-CBS-YPet (CNNM3 Synthetic, Human)
    

    Tags

    8 histidine tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 23, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCKO_EIF3D_sgRNA_1

    Plasmid

    #74187

    Purpose

    lentiviral vector expressing sgRNA targeting EIF3D

    Depositor

    Jason Moffat

    Article

    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10

    Insert

    EIF3D sgRNA
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 Promoter

    Available Since

    March 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. LXSN-Cx43

    Plasmid

    #60692

    Purpose

    retroviral expression of rat Cx43

    Depositor

    Parmender Mehta

    Article

    Johnson et al Mol Biol Cell. 2013 Mar;24(6):715-33. do

    Insert

    Cx43 (Gja1 Rat)
    

    Use

    Retroviral

    Expression

    Mammalian

    Promoter

    LTR

    Available Since

    July 6, 2015

    Availability

    Academic Institutions and Nonprofits only

20. Sema7a-AP-His

    Plasmid

    #72049

    Purpose

    Expresses the extracellular region of the Sema7A protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

    Depositor

    Woj Wojtowicz

    Article

    Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

    Insert

    Sema7a (Sema7a Mouse)
    

    Tags

    AP-His

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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