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We narrowed to 14,203 results for: cas9

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Showing: 11181 - 11200 of 14203 results

  1. tRNA-gRNA position [M1_2] (GB1209)

    Plasmid
    #75410
    Purpose
    tRNA and scaffold for the assembly of GBoligomers for the first position (positon [M1_2]) of a polycistronic tRNA-gRNA regulated by the (monocot) U3 promoter (3-part multiplexing)
    Depositor
    Diego Orzaez
    Publication
    Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10
    Insert
    tRNA-gRNA position [M1_2]
    Use
    CRISPR and Synthetic Biology
    Expression
    Plant
    Available since
    Aug. 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    75410-plasmid-map-sequence-id-146027

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  5. HCP6

    Plasmid
    #166108
    Purpose
    This plasmid encodes a Cas9 protein as well as two sgRNAs, one targets the C-terminus of Cyr1 and the other targets a non-coding region on chromosome X (location X1)
    Depositor
    Matthias Heinemann
    Insert
    Cyr1CT-sgRNA/X1-sgRNA (CYR1 Budding Yeast)
    Use
    CRISPR
    Expression
    Yeast
    Available since
    April 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    166108-plasmid-map-sequence-id-330318

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  9. pKL2013

    Plasmid
    #172182
    Purpose
    A CRISPR/Cas9 vector targeting maize glossy2 gene for targeted mutagenesis with a mCherry as fluorescent marker.
    Depositor
    Kan Wang
    Publication
    McCaw et al Front Genome Ed. 2021 Jan 11;2:622227. d
    Insert
    mCherry
    Use
    CRISPR
    Expression
    Plant
    Promoter
    Cauliflower mosaic virus 35S promoter
    Available since
    Aug. 4, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    172182-plasmid-map-sequence-id-339004

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  10. pJ204-loxP-EF1a-mCherry-NeoR-lox2272-BGHpA-siteA-HDR

    Plasmid
    #154826
    Purpose
    Landing pad for Cre/lox-based recombinase-mediated cassette exchange (RMCE) in CHO cells, donor plasmid for CRISPR/Cas9-mediated targeted integration in CHO cells (site A)
    Depositor
    Lise Marie Grav
    Publication
    Sergeeva et al ACS Synth Biol. 2020 Aug 24. doi: 10.102
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Inserts
    mCherry
    NeoR
    ZsGreen1-DR
    Use
    CRISPR and Cre/Lox
    Tags
    PEST
    Expression
    Mammalian
    Promoter
    CMV, EF1a, and SV40 early promoter
    Available since
    Aug. 25, 2020
    Availability
    Academic Institutions and Nonprofits only
    154826-plasmid-map-sequence-id-306204

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  13. pRPCE

    Plasmid
    #193712
    Purpose
    Expresses cytosine deaminase linked deactivated-Cas9 from RK2 origin with RFP-marked sgRNA insertion site and kanR/sacB selection/counterselection
    Depositor
    Martin Buck
    Type
    Empty backbone
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Available since
    Feb. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    193712-plasmid-map-sequence-id-381728

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  18. pODN-AAVS1-mNG-CAAX

    Plasmid
    #183870
    Purpose
    Repair template for the stable expression of a mNeonGreen-CAAX fusion in human cells using CRISPR/Cas9.
    Depositor
    Alisa Piekny
    Publication
    Husser et al Open Biol. 2022 Nov;12(11):220247. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    AAVS1 homology arms with CMV-driven mNeonGreen-CAAX fusion (AAVS1 Human)
    Use
    CRISPR; Donor template
    Tags
    mNeonGreen-CAAX
    Expression
    Mammalian
    Mutation
    Homology arms contain point mutations to remove t…
    Promoter
    CMV
    Available since
    May 20, 2022
    Availability
    Academic Institutions and Nonprofits only
    183870-plasmid-map-sequence-id-362419

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Showing: 11181 - 11200 of 14203 results