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pSG5-HA-CaMKKbeta rat FL WT

Plasmid

#33321

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

HA

Expression

Mammalian

Mutation

S3V, G96R, and P108L

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
pSG5-FLAG-CaMKKbeta rat S129D, S133D, S137D

Plasmid

#32457

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

S129D, S133D, S137D (S3V, G96R, and P108L also pr…

Promoter

SV40

Available since

April 17, 2012

Availability

Academic Institutions and Nonprofits only
Lentiviral FLAG-CaMKKbeta S129D, S133D, S137D

Plasmid

#32460

Depositor

Anthony Means

Publication

Green et al J Biol Chem. 2011 Aug 12;286(32):28066-7

Insert

Calcium/Calmodulin dependent protein kinase kinase beta (Camkk2 Mouse)

Use

Lentiviral

Tags

FLAG

Mutation

S129D, S133D, S137D

Promoter

human PGK

Available since

Feb. 7, 2012

Availability

Academic Institutions and Nonprofits only
pYPQ135-tRNA2.0

Plasmid

#158397

Purpose

Golden Gate entry vector to express the 5th gRNA with tRNA2.0 scaffold (with four MS2 binding sites) without promoter

Depositor

Yiping Qi

Publication

Pan et al Nat Plants. 2021 Jul;7(7):942-953. doi:

Type

Empty backbone

Use

CRISPR

Expression

Plant

Promoter

Without promoter

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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mIFP-Calnexin-N-14

Plasmid

#56214

Purpose

Localization: Endoplasmic Reticulum, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

Calnexin (CANX Human)

Tags

mIFP

Expression

Mammalian

Mutation

I474N in Calnexin

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

Cited by

1 Article
mIFP-Lysosomes-20

Plasmid

#56228

Purpose

Localization: Lysosome Membrane, Excitation: 683, Emission: 704

Depositor

Michael Davidson

Publication

Yu et al Nat Methods. 2015 Aug;12(8):763-765. doi

Insert

LAMP1

Tags

mIFP

Expression

Mammalian

Promoter

CMV

Available since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
pSIN4-HCK

Plasmid

#254425

Purpose

Mammalian expression plasmid encoding human HCK fused to a 3_FLAG tag.

Depositor

Rui Su

Publication

Ren et al Blood. 2026 Jun 18;147(25):3069-3085. do

Insert

HCK (HCK Human)

Use

Lentiviral

Tags

3_FLAG

Promoter

EF-1alpha

Available since

April 9, 2026

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.3 N-MYC FLNA

Plasmid

#227876

Purpose

Used for transfection of cells for expression of C-MYC FLNA

Depositor

Sheng-cai Lin

Publication

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

FLNA (Flna Mouse)

Tags

MYC

Expression

Mammalian

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a KGA-WT

Plasmid

#227846

Purpose

To express in E. coli KGA-WT

Depositor

Sheng-cai Lin

Publication

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

KGA isoform (GLS Human)

Tags

HIS

Expression

Bacterial

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pET-28a GAC-WT

Plasmid

#227845

Purpose

To express in E. coli GAC-WT

Depositor

Sheng-cai Lin

Publication

Li et al Cell Res. 2024 Jun 19. doi: 10.1038/s414

Insert

GAC isoform (GLS Human)

Tags

HIS

Expression

Bacterial

Available since

June 27, 2025

Availability

Academic Institutions and Nonprofits only

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pEAHISPApqsE

Plasmid

#97019

Purpose

Expressing Pseudomonas aeruginosa pqsE protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Quinolone signal response protein

Tags

N-ter TEV protease cleavable 6HIs

Mutation

none

Promoter

T7

Available since

Aug. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2676

Plasmid

#96970

Purpose

expression of methicillin-resistant Staphylococcus aureus orf 2676

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Sar2676

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

Aug. 7, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSA2028

Plasmid

#97001

Purpose

Expressing MRSA Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Asp/Tyr/Phe pyridoxal-50- phosphate-dependent aminotransferase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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pEAHISMRSAMVAK

Plasmid

#97000

Purpose

expression of methicillin-resistant Staphylococcus aureus MVAK protein

Depositor

Huanting Liu, James Naismith

Publication

Oke et al J Struct Funct Genomics. 2010 Jun;11(2):

Insert

Mevalonate kinase

Tags

N-ter TEV protease cleavable 6His

Mutation

none

Promoter

T7

Available since

July 19, 2017

Availability

Academic Institutions and Nonprofits only

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TJF229

Plasmid

#132575

Purpose

pZE1-oxySp-mCherry-proD-oxyR

Depositor

Timothy Lu

Publication

Muller et al Nat Commun. 2019 Sep 6;10(1):4028. doi:

Inserts

oxySp-mCherry-TermT1
proD-oxyR-TermT1

Expression

Bacterial

Promoter

oxySp and proD

Available since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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pRS316-IVb

Plasmid

#31457

Depositor

Ana Kitazono

Publication

Kitazono et al Gene. 2011 Sep 15;484(1-2):86-9. doi: 10

Insert

linker

Expression

Yeast

Available since

Aug. 29, 2011

Availability

Academic Institutions and Nonprofits only
CAG FLEX BARK D110A p2A mCherry

Plasmid

#117694

Purpose

CAG-FLEx-iBARK(D110A)-p2A-mCherry: Negative control viral expression vector for Cre-dependent Gaq silencing

Depositor

Baljit Khakh

Publication

Nagai et al Neuron. 2021 Jul 21;109(14):2256-2274.e9

Insert

BARKrgs D110A peptide

Use

Cre/Lox

Tags

HA / FLAG

Mutation

D110A

Available since

May 28, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

Plasmid

#169912

Purpose

Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

Depositor

Zhili Rong

Publication

Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

Insert

dCjCas9

Use

CRISPR

Tags

10XGCN4, 2xSV40NLS, and SV40NLS-HA

Expression

Mammalian

Mutation

D8A, H559A in cjCas9

Promoter

CMV

Available since

June 25, 2021

Availability

Academic Institutions and Nonprofits only

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pMSCV-FKBP-IRF3

Plasmid

#221260

Purpose

Expression of IRF3 in mammalian cells by retroviral transduction

Depositor

Jonathan Kagan

Publication

Landau et al Mol Cell. 2024 Jul 11;84(13):2436-2454.e

Insert

IRF3 (Irf3 Mouse)

Use

Retroviral

Tags

3xFLAG-4xFKBP

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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CMV-dCasRx-ccdB

Plasmid

#221003

Purpose

Destination vector for Gateway cloning of ORFs into the C-terminus of dCasRx. CMV promoter. Transient expression.

Depositor

Benjamin Blencowe, Mikko Taipale

Publication

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available since

June 24, 2024

Availability

Academic Institutions and Nonprofits only

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