We narrowed to 19,741 results for: Cre

1. His6tev CBP BCHZ (1079-1751)

   Plasmid

   #99341

   Purpose

   Bacterial expression of His tagged mouse CBP HAT core Brd-ZZ (1079-1751)

   Depositor

   Peter Wright

   Article

   Park et al Proc Natl Acad Sci U S A. 2017 Jun 19. p

   Insert

   mouse CBP BRD-ZZ domains (1079-1751) (Crebbp Mouse)
   

   Tags

   His6tev

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Sept. 15, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. BDKBR1-DuET

   Plasmid

   #213189

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   BDKBR1 (BDKRB1 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1.H1-NP

   Plasmid

   #134367

   Purpose

   Nanoluc complementation assay. Expression of histamine receptor H1 fused at C termimus with Natural peptide (NP) of NanoLuc. Addition of the Flag epitope at N terminus of H1 receptor.

   Depositor

   Julien Hanson

   Article

   Laschet et al J Biol Chem. 2019 Mar 15;294(11):4079-40

   Insert

   H1-NP (HRH1 Human)
   

   Tags

   Flag and natural peptide of nanoluciferase

   Expression

   Bacterial and Mammalian

   Promoter

   T7

   Available Since

   July 22, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. LV_EF1a_MAPK14-P2A-Hygro_Barcode

   Plasmid

   #170239

   Purpose

   Barcoded lentiviral vector to express MAPK14 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAPK14 (MAPK14 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. PARP1-c009

   Plasmid

   #216872

   Purpose

   Bacterial expression vector for E. coli producing PARP1 catalytic protein (without Regulatory domain); with N-terminal CFP fusion

   Depositor

   Lari Lehtiö

   Article

   Dhakar et al Sci Rep. 2024 Feb 16;14(1):3875. doi: 10

   Insert

   PARP1 catalytic domain (no Regulatory Domain) (PARP1 Human)
   

   Expression

   Bacterial

   Available Since

   May 23, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. EF1a_KLF5_P2A_Hygro_Barcode

   Plasmid

   #120491

   Purpose

   Barcoded lentiviral vector to express KLF5 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

   Depositor

   Prashant Mali

   Article

   Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

   Insert

   KLF5 (KLF5 Human)
   

   Use

   Lentiviral

   Promoter

   EF1a

   Available Since

   Feb. 6, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCMV_UdgX-EE-UdgX-NG-nCas9-UdgX

   Plasmid

   #163572

   Purpose

   Mammalian CG-to-GC base editing

   Depositor

   David Liu

   Article

   Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

   Insert

   UdgX-EE-UdgX-NG-nCas9-UdgX
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   nCas9 (D10A)EE (R126E, R132E)NG (L111R, D1135V, G…

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Dom neg CstF64

   Plasmid

   #127250

   Purpose

   Expresses the Dominant Negative CstF64 in mammalian cells; DN blocks polyadenylation

   Depositor

   Christine Milcarek

   Article

   Martincic et al Nat Immunol. 2009 Oct;10(10):1102-9. doi

   Insert

   CstF64 delta 282 (CSTF2 Human)
   

   Use

   Other

   Expression

   Mammalian

   Mutation

   insert @SanD1:GTCCAGGCGCCTACCCATACGACGTCCCAGACTAC…

   Promoter

   pEF1a

   Available Since

   July 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. HTR6-DuET

   Plasmid

   #213326

   Purpose

   The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.

   Depositor

   Thomas Sakmar

   Article

   Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:

   Insert

   HTR6 (HTR6 Human)
   

   Tags

   1D4 and FLAG

   Expression

   Mammalian

   Promoter

   CMV, T7

   Available Since

   April 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. TKTL1

    Plasmid

    #72419

    Purpose

    Study the role of aberrant expression of TKTL1 in HNSCC tumorigenesis

    Depositor

    David Sidransky

    Article

    Sun et al Clin Cancer Res. 2010 Feb 1;16(3):857-66

    Insert

    TKTL1 (TKTL1 Human)
    

    Expression

    Mammalian

    Mutation

    may have one or two synonymous substitutions

    Promoter

    CMV

    Available Since

    April 22, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pDONR221 MLK1 E179K

    Plasmid

    #60541

    Purpose

    Donor vector for human MLK1 E179K mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Mutation

    E179K

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

12. Str-STIM1-NN_puromycin

    Plasmid

    #65310

    Purpose

    Luminal ER hook only (RUSH system)

    Depositor

    Franck Perez

    Article

    Boncompain et al Nat Methods. 2012 Mar 11;9(5):493-8. doi

    Insert

    STIM1 mutated for binding motif to microtubule (STIM1 Human)
    

    Tags

    streptavidin

    Expression

    Mammalian

    Mutation

    2 amino acids of STIM1 (IP from SxIP motif) were …

    Promoter

    CMV

    Available Since

    June 19, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR221 MLK1 D294A

    Plasmid

    #60542

    Purpose

    Donor vector for human MLK1 D294A mutant

    Depositor

    John Brognard

    Article

    Fawdar et al Proc Natl Acad Sci U S A. 2013 Jul 23;11

    Insert

    MLK1 (MAP3K9 Human)
    

    Use

    Gateway donor vector

    Mutation

    D294A

    Available Since

    Feb. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pT7-G-LbCas12a crRNA-BsaI cassette (MSP3495)

    Plasmid

    #114068

    Purpose

    T7 promoter crRNA entry vector used for in vitro transcription of LbCas12a guides

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    entry vector for LbCas12a crRNAs
    

    Use

    In vitro transcription

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pAct-FRT-FRT3-stop-FRT-LexGAD-FRT3-Gal4 attB

    Plasmid

    #52890

    Purpose

    "CoinFLP-LexGAD/Gal4" - Expresses LexGAD or Gal4 from actin promoter downsteam of transcriptional stop. Recombination by FLP excises FRT-FRT pair to express LexGAD, or FRT3-FRT3 pair to express Gal4.

    Depositor

    Iswar Hariharan

    Article

    Bosch et al Development. 2015 Feb 1;142(3):597-606.

    Inserts

    LexGAD
    Gal4 (GAL4 Budding Yeast)
    

    Expression

    Insect

    Available Since

    Jan. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTRIP TRE-BI GFP10-RHOB/RBD-GFP11

    Plasmid

    #182231

    Purpose

    Inducible coexpression of GFP10-RHOB and RBD-GFP11 domain

    Depositor

    Stéphanie Cabantous

    Article

    Koraichi et al J Cell Sci. 2018 Jan 8;131(1). pii: jcs.

    Inserts

    ras homolog family member B (RHOB Human)
    rhotekin RHO binding domain (RBD)
    

    Use

    Lentiviral

    Tags

    GFP10 (split-GFP tripartite) and GFP11 (M4)

    Promoter

    TRE bidirectional

    Available Since

    April 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pXPR_003 sgSMARCA4 guide 2

    Plasmid

    #193605

    Purpose

    SMARCA4 knockout

    Depositor

    William Hahn

    Article

    Hagel et al Cancer Res. 2022 Dec 22:CAN-22-2245. doi

    Insert

    sgSMARCA4 guide 2 (SMARCA4 Human)
    

    Use

    Lentiviral

    Available Since

    Jan. 3, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. EF1a_KLF13_P2A_Hygro_Barcode

    Plasmid

    #120499

    Purpose

    Barcoded lentiviral vector to express KLF13 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    KLF13 (KLF13 Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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19. EF1a_MEF2C_P2A_Hygro_Barcode

    Plasmid

    #120458

    Purpose

    Barcoded lentiviral vector to express MEF2C in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MEF2C (MEF2C Human)
    

    Use

    Lentiviral

    Promoter

    EF1a

    Available Since

    Feb. 5, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEX-CDC27 wt

    Plasmid

    #31673

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    cell division cycle 27 homolog (CDC27 Human)
    

    Tags

    GST

    Expression

    Bacterial

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only