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pLV-eGFP

Plasmid

#36083

Purpose

3rd generation lentiviral transfer plasmid

Depositor

Pantelis Tsoulfas

Article

Enomoto et al Exp Neurol. 2013 Oct;248:170-82. doi: 10

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Available Since

June 1, 2012

Availability

Academic Institutions and Nonprofits only

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pDe-CAS9

Plasmid

#61433

Purpose

Binary expression vector with A.th. codon-optimized Cas9 and Gateway destination sequence

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

Cas9

Use

CRISPR

Expression

Plant

Promoter

PcUBI4-2

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP-150TAG

Plasmid

#174076

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP-150 TAG with C-terminal His6 tag, under T7 promoter,

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Mutation

N150TAG

Promoter

T7

Available Since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti-Tet

Plasmid

#138086

Purpose

Empty vector with Tet-response promoter for inducible expression of proteins. Can be transduced using Lentiviral particles

Depositor

Eva Frickel

Article

Fisch et al EMBO J. 2019 Jul 1;38(13):e100926. doi:

Type

Empty backbone

Use

Lentiviral

Expression

Mammalian

Promoter

minimal CMV promoter with heptamerized tet‐operat…

Available Since

March 30, 2020

Availability

Academic Institutions and Nonprofits only

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cmv-sfGFP-GB1-scAB-ubc-nls-pcp-5xSunTag(SRv1-2P)

Plasmid

#185800

Purpose

one plasmid encoding scFv GFP and pcp-5xSunTag components for SunRISER SRv1

Depositor

Robin Lee

Article

Guo et al Cell Rep Methods. 2022 May 25;2(6):10022

Insert

cmv-sfGFP-GB1-scAB--ubc-nls-pcp-5xSunTag

Expression

Mammalian

Available Since

July 18, 2022

Availability

Academic Institutions and Nonprofits only

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pGL6000

Plasmid

#216797

Purpose

Platform for DiCre inducible expression of a gene of interest in Leishmania major - requires Leishmania strain expressing DiCre recombinase

Depositor

Jeremy Mottram

Article

Santos et al Mol Biochem Parasitol. 2017 Sep;216:45-4

Type

Empty backbone

Use

Cre/Lox; Leishmania dicre inducible expression

Tags

6xHA

Promoter

rRNA promoter

Available Since

June 20, 2024

Availability

Academic Institutions and Nonprofits only

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pMJ806

Plasmid

#39312

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Promoter

T7

Available Since

Sept. 7, 2012

Availability

Academic Institutions and Nonprofits only

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pMJ825

Plasmid

#39315

Depositor

Jennifer Doudna

Article

Jinek et al Science. 2012 Aug 17;337(6096):816-21. d

Insert

Cas9

Use

CRISPR

Tags

His6 and MBP

Expression

Bacterial

Mutation

Aspartate 10 to Alanine (D10A)

Promoter

T7

Available Since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only

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pUC57-Kan_BR29_00121041

Plasmid

#121233

Purpose

Level 0 Golden-Gate compatible vector

Depositor

Sophien Kamoun

Article

Petit-Houdenot et al Mol Plant Microbe Interact. 2020 Aug;33(

Insert

BR29_00121041

Use

Golden gate

Mutation

Magnaporthe grisea

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1-T2A-tdTomato

Plasmid

#60598

Purpose

Expresses Kir2.1-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kir2.1-T2A-tdTomato (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Available Since

Nov. 10, 2014

Availability

Academic Institutions and Nonprofits only

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pCS-APTA

Plasmid

#247077

Purpose

pCS27 containing aroL, ppsA, tktA, aroG(fbr) from E. coli

Depositor

Yajun Yan

Article

Shen et al Metab Eng. 2017 Jul;42:52-58. doi: 10.10

Inserts

aroL
ppsA
tktA
aroG(fbr)

Expression

Bacterial

Mutation

feedback-inhibition-resistant

Promoter

PLlacO1

Available Since

Oct. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-H2B-miRFP670

Plasmid

#212668

Purpose

Express tagged H2B

Depositor

Yumi Konagaya, Tobias Meyer

Article

Konagaya et al Nature. 2024 Jul;631(8020):424-431. doi:

Insert

H2B (H2BC11 Human)

Use

Lentiviral

Tags

miRFP670

Promoter

EF-1a

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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pCAG-Kir2.1Mut-T2A-tdTomato

Plasmid

#60644

Purpose

Expresses Kir2.1Mut-T2A-tdTomato

Depositor

Massimo Scanziani

Article

Xue et al Nature. 2014 Jul 31;511(7511):596-600. d

Insert

Kcnj2 (Kcnj2 Mouse)

Tags

myc

Expression

Mammalian

Mutation

GYG to AAA (aa144-146)

Available Since

Oct. 27, 2014

Availability

Academic Institutions and Nonprofits only

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EF1a-dCasRx-RBM25

Plasmid

#221001

Purpose

Transient expression of dCasRx-RBM25. EF1a promoter.

Depositor

Benjamin Blencowe, Mikko Taipale

Article

Li et al Mol Cell. 2024 Jul 11;84(13):2573-2589.e

Insert

dCasRx-RBM25

Use

CRISPR

Tags

NLS and NLS-HA

Expression

Mammalian

Promoter

EF1a

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFPc1 Rab23

Plasmid

#237337

Purpose

transient overexpression in mammalian cells

Depositor

Michele Pagano

Article

Kuchay et al Nat Struct Mol Biol. 2019 Jul;26(7):628-

Insert

Rab23 (RAB23 Mouse)

Expression

Mammalian

Available Since

May 21, 2025

Availability

Academic Institutions and Nonprofits only

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pHisIHFαIHFβ

Plasmid

#149384

Purpose

Expression of P. aeruginosa PA14, 6xHis-HRV3C-IHFalpha and IHFbeta

Depositor

Blake Wiedenheft

Article

Santiago-Frangos et al Curr Biol. 2021 Aug 23;31(16):3515-3524.

Inserts

Pseudomonas aeruginosa PA14 6xHis-tagged IHFalpha
Pseudomonas aeruginosa PA14 IHFbeta

Tags

6xHis tag and HRV3C cleavage site

Expression

Bacterial

Promoter

T7

Available Since

Aug. 23, 2022

Availability

Academic Institutions and Nonprofits only

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SS-HA-Sun1L-KDEL

Plasmid

#187016

Purpose

Disruption of LINC Complex

Depositor

Jan Lammerding

Article

Lombardi et al J Biol Chem. 2011 Jul 29;286(30):26743-5

Insert

SS-HA-Sun1L-KDEL

Use

Lentiviral

Available Since

Aug. 5, 2022

Availability

Academic Institutions and Nonprofits only

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pChimera

Plasmid

#61476

Purpose

Vector for conventional cloning that contains AtU6-26 promoter and sgRNA backbone

Depositor

Holger Puchta

Article

Fauser et al Plant J. 2014 Jul;79(2):348-59. doi: 10.

Insert

U6-26:sgRNA

Use

CRISPR

Expression

Plant

Promoter

U6-26

Available Since

Sept. 24, 2018

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab9a

Plasmid

#78592

Purpose

Express mCherry-Rab9a in mammalian cells

Depositor

Yihong Ye

Article

Lee et al Nat Cell Biol. 2016 Jul;18(7):765-76. do

Insert

Rab9a (RAB9A Human)

Tags

mCherry2

Expression

Mammalian

Promoter

CMV

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only

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pRBC-sfGFP wild type

Plasmid

#174075

Purpose

Plasmid with p15a origin of replication expressing codon optimized superfolder GFP with C-terminal His6 tag, under T7 promoter

Depositor

Ryan Mehl

Article

Zhu et al ACS Chem Biol. 2019 Jul 19;14(7):1564-15

Insert

Superfolder GFP

Tags

His-6

Expression

Bacterial

Promoter

T7

Available Since

Oct. 20, 2021

Availability

Academic Institutions and Nonprofits only

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