We narrowed to 19,744 results for: Cre

1. pRD440

   Plasmid

   #168466

   Purpose

   For the insertion pf NLS-mCherry-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mCherry-H-NSdbd-NLS
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRD441

   Plasmid

   #168467

   Purpose

   For the insertion pf NLS-mEGFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mEGFP-H-NSdbd-NLS
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pRD442

   Plasmid

   #168468

   Purpose

   For the insertion of NLS-eYFP-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-eYFP-H-NSdbd-NLS
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pRD443

   Plasmid

   #168469

   Purpose

   For the insertion pf NLS-mScarlet-I-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mScarlet-I-H-NSdbd-NLS
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pRD444

   Plasmid

   #168470

   Purpose

   For the insertion of NLS-mNeonGreen-H-NSdbd-NLS into the chromosome of a eukaryotic cell line carrying the Flp-IN/T-Rex system

   Depositor

   Remus Dame

   Article

   Rashid et al Nucleic Acids Res. 2021 Nov 4. pii: 6414

   Insert

   NLS-mNeonGreen-H-NSdbd-NLS
   

   Expression

   Mammalian

   Promoter

   CMV promoter

   Available Since

   Oct. 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. LV_EF1a_MAPK9-P2A-Hygro_Barcode

   Plasmid

   #170238

   Purpose

   Barcoded lentiviral vector to express MAPK9 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq

   Depositor

   Prashant Mali

   Article

   Parekh et al iScience. 2021 Sep 20;24(10):103149. doi

   Insert

   MAPK9 (MAPK9 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Promoter

   EF1a

   Available Since

   Sept. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. EGFP-uTEV1-MAVS-mTA6

   Plasmid

   #171004

   Purpose

   HiLITR protease with MAVS [Y536delinsFIVLI, R537A, R538A] C-terminal tmd targeting information (Mitochondria)

   Depositor

   Alice Ting

   Article

   Coukos et al eLife. 2021 Aug 20;10. pii: 69142. doi:

   Insert

   EGFP-uTEV1-MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]
   

   Use

   Lentiviral and Synthetic Biology

   Expression

   Mammalian

   Mutation

   MAVS(tmd)[Y536delinsFIVLI, R537A, R538A]

   Promoter

   pTRE-tight (TetOn)

   Available Since

   Aug. 19, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pInducer10-UBC9-sh1

   Plasmid

   #168986

   Purpose

   Expresses RFP cDNA and UBC9 shRNA 1 in mammalian cells

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   Ubiquitin Conjugating Enzyme 9 (UBE2I )
   

   Expression

   Mammalian

   Promoter

   Ubc promoter

   Available Since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pDECKO_mCherry_KLF4

   Plasmid

   #140104

   Purpose

   CRISPR-Cas9 library validation

   Depositor

   Roderic Guigo

   Article

   Arnan et al BMC Genomics. 2022 May 26;23(1):402. doi

   Insert

   gRNA1+scaffold+H1promoter+gRNA2
   

   Use

   Lentiviral

   Promoter

   gRNA1 under U6 and gRNA2 under H1

   Available Since

   April 29, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. CPH3506 (pET151_Spt6 1247-1451 K1355A,K1435A)

    Plasmid

    #91818

    Purpose

    Bacterial expression of residues 1247-1451 from S. cerevisiae Spt6 with K1435A and K1355A mutations

    Depositor

    Chris Hill

    Article

    Sdano et al Elife. 2017 Aug 16;6. pii: 28723. doi: 1

    Insert

    SPT6 (SPT6 Budding Yeast)
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    changed lysine 1355 and lysine 1435 to alanines; …

    Promoter

    T7

    Available Since

    March 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCMV_RBMX-eA3A-UdgX-HF-NG-nCas9

    Plasmid

    #163550

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    RBMX-eA3A-UdgX-HF-NG-nCas9
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)N497A, R661A, Q695A, Q926A//L111R, D1…

    Available Since

    Dec. 22, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. LB001: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#2 gRNA

    Plasmid

    #131757

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. LA901: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::Sp/xCas9 TRE#1 gRNA

    Plasmid

    #131756

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven Sp/xCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 Sp/xCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. JV201: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#1 gRNA

    Plasmid

    #131755

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#1 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#1 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#1 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 15, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. LB101: pMAGIC (L3-L2) 3x HA epitope tag + polyA; hU6::SaCas9 TRE#2 gRNA

    Plasmid

    #131758

    Purpose

    pMAGIC L3-L2 entry plasmid, contains 3xHA tag+polyA; hU6-driven SaCas9 TRE#2 gRNA for 3-/4-component MultiSite Gateway Pro assembly. Allows C-term HA epitope fusion to dCas9 w/ TRE#2 gRNA expression

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Insert

    TRE#2 SaCas9 gRNA
    

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Promoter

    hU6

    Available Since

    Oct. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. nSaCas9 P742 miniABEMax V82G

    Plasmid

    #135385

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (P742 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 P742 miniABEMax V82G
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. nSaCas9 Q731 miniABEMax V82G

    Plasmid

    #135386

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q731 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 Q731 miniABEMax V82G
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. nSaCas9 Q737 miniABEMax V82G

    Plasmid

    #135387

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (Q737 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 Q737 miniABEMax V82G
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. nSaCas9 S740 miniABEMax V82G

    Plasmid

    #135388

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (S740 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 S740 miniABEMax V82G
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. nSaCas9 T128 miniABEMax V82G

    Plasmid

    #135389

    Purpose

    Expression of nSaCas9 intradomain insertion of single evolved TadA monomer (V82G) at the specified position (T128 )

    Depositor

    Alex Hewitt

    Article

    Nguyen Tran et al Nat Commun. 2020 Sep 25;11(1):4871. doi:

    Insert

    nSaCas9 T128 miniABEMax V82G
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    (V82G)

    Available Since

    Oct. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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