We narrowed to 20,250 results for: Cre
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Plasmid#138679PurposeExpresses a mouse SIK1-targeting sgRNA and Cas9DepositorAvailable SinceFeb. 26, 2020AvailabilityAcademic Institutions and Nonprofits only
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hsa-cdk2 3'UTR sicheck2 mut
Plasmid#121124Purposeluciferase assayDepositorInserthsa-cdk2 3'UTR mutated 199 binding (CDK2 Human)
ExpressionBacterialMutationmiR-199 seed binding site mutatedAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
Tol2-lyzC-mcherry-2a-CDK2-D145N
Plasmid#121132Purposeneutrophil specific expressionDepositorInsertdre-D145N CDK2 DN (cdk2 Zebrafish)
Tagsmcherry-2aExpressionBacterialMutationD145N point mutation DNPromoterneutrophil specific lyzCAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
dre-cdk2 3'UTR sicheck2 199 mut
Plasmid#121122Purposeluciferase assayDepositorInsertdre-cdk2 3'UTR mutated 199 binding (cdk2 Zebrafish)
ExpressionBacterialMutationmiR-199 seed binding site mutatedAvailable SinceSept. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Slc25a51-g1)-PGKpuroBFP-W
Plasmid#105028PurposeLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Slc25a51-g2)-PGKpuroBFP-W
Plasmid#105029PurposeLentiviral gRNA plasmid targeting mouse Slc25a51 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Pou5f1-g1)-PGKpuroBFP-W
Plasmid#105035PurposeLentiviral gRNA plasmid targeting mouse Pou5f1 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Kcmf1-g1)-PGKpuroBFP-W
Plasmid#105017PurposeLentiviral gRNA plasmid targeting mouse Kcmf1 , co-expression of TagBFPDepositorAvailable SinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
289aa Met133, 138, 186Ileu ELL2
Plasmid#127272PurposeExpresses 289aa human ELL2 with Met133, 138, 186IleuDepositorInsertELL2 w Met to ILeus stopped at XbaI (ELL2 Human)
ExpressionMammalianMutationMet133, 138, 186 Ileu, cut with XbaI blunt fillinPromoterpEF1aAvailable SinceJuly 15, 2019AvailabilityAcademic Institutions and Nonprofits only -
frameshift ELL2
Plasmid#127257PurposeFor in vitro translation of human a frameshift ELL2 that results in a 58 kDa product from internal initiation.DepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationXhoI site between the 1st &2nd Met was filled…PromoterT7Available SinceJuly 12, 2019AvailabilityAcademic Institutions and Nonprofits only -
564aa ELL2 bluescript
Plasmid#127267PurposeFor in vitro translation of human ELL2 lacking COOH regionDepositorInsertELL2 (ELL2 Human)
UseIn vitro translationMutationAddgene Plasmid 127256--SphI site filled in, then…PromoterT7Available SinceJuly 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA901: pMVP (L3-L2) HA tag + pA; EF1a::eGFP-P2A-TETa-pA
Plasmid#121804PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + EF1a-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream EF1a-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + EF1a::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KA701: pMVP (L3-L2) HA tag + pA; RIP::eGFP-P2A-TETa-pA
Plasmid#121808PurposepMVP L3-L2 entry plasmid, contains HA tag-polyA + RIP-eGFP-P2A-TETa-polyA for 3- or 4-component MultiSite Gateway Pro assembly. Adds C-term HA tag to gene plus downstream RIP-driven GFP-P2A-TETaDepositorInsertHA epitope tag-polyA + RIP::eGFP-P2A-TETa-polyA
UseSynthetic Biology; Pmvp gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
KB701: pMAGIC (L5-L4) hU6::SaCas9 gRNA scaffold; CMV promoter
Plasmid#121818PurposepMAGIC L5-L4 entry plasmid, contains empty human U6-driven SaCas9 gRNA scaffold and CMV promoter for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestionDepositorTypeEmpty backboneUseSynthetic Biology; Pmagic gateway entry plasmidAvailable SinceJune 27, 2019AvailabilityAcademic Institutions and Nonprofits only -
EF1a_KLF8_P2A_Hygro_Barcode
Plasmid#120494PurposeBarcoded lentiviral vector to express KLF8 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.DepositorAvailable SinceFeb. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFUSEss-CHIg-mG3_CH2charge
Plasmid#105858PurposeExpression plasmid coding for mutated heavy chain of mouse IgG3 antibody specific to antigen B of the ABO blood group system, clone M18. Introduced mutatnions modify charge of CH2 domain.DepositorInsertIgG3 heavy chain (Ighg3 Mouse)
ExpressionMammalianMutationmutated heavy chain of mouse IgG3, muatations: Hi…PromoterhEF1-HTLV promAvailable SinceMarch 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-Notch1 3'UTR-mir200c-mut
Plasmid#84596PurposeRenilla Luciferase reporter assay for NOTCH1 3'UTR with mutations on miR-200c binding siteDepositorInsertNOTCH1 3'UTR (NOTCH1 Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on NOTCH1 3…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCIneoRL-LFNG 3'UTR-mir200c-mut
Plasmid#84594PurposeRenilla Luciferase reporter assay for LFNG 3'UTR with mutations on miR-200c binding siteDepositorInsertLFNG 3'UTR (LFNG Human)
UseLuciferaseTagsRenilla LuciferaseMutationMutation on miR-200c binding site on LFNG 3'…PromoterCMVAvailable SinceNov. 4, 2016AvailabilityAcademic Institutions and Nonprofits only -
Lrrc4c-AP-His
Plasmid#71959PurposeExpresses the extracellular region of the LRRC4C protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.DepositorAvailable SinceJan. 25, 2016AvailabilityAcademic Institutions and Nonprofits only -
LXSN-Cx43-S279A
Plasmid#60705Purposeretroviral expression of rat Cx43 S279A mutantDepositorAvailable SinceJuly 6, 2015AvailabilityAcademic Institutions and Nonprofits only