We narrowed to 19,744 results for: Cre

1. Flrt3-Fc-His

   Plasmid

   #72075

   Purpose

   Expresses the extracellular region of the FLRT3 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Flrt3 (Flrt3 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. TetO-FUW-Mecom 1

   Plasmid

   #118590

   Purpose

   Expresses Mecom isoform 1 from the TRE3G promoter

   Depositor

   Stanley Qi

   Article

   Liu et al Cell Stem Cell. 2018 Sep 28. pii: S1934-

   Insert

   Mecom (Mecom Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   TRE3G

   Available Since

   Nov. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA5FRT/TO-Ataxin3-Strep-HA

   Plasmid

   #113496

   Purpose

   Expression of human Ataxin-3 with C-terminal strep-HA tag

   Depositor

   Hemmo Meyer

   Article

   Hulsmann et al Mol Cell Proteomics. 2018 Mar 29. pii: R

   Insert

   Ataxin-3 (ATXN3 Human)
   

   Tags

   2xstrep-HA

   Expression

   Mammalian

   Mutation

   wildtype without stop codon

   Promoter

   CMV

   Available Since

   Aug. 15, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAGGS-SARS2-S-FKO (C-flag)

   Plasmid

   #159364

   Purpose

   Mammalian expression of SARS-CoV-2 S protein (FKO: furin-cleavage site mutant) with the flag tag at C-terminus.

   Depositor

   Hyeryun Choe, Michael Farzan

   Article

   Zhang et al Nat Commun. 2020 Nov 26;11(1):6013. doi:

   Insert

   SARS-CoV-2 S protein (S SARS-CoV-2)
   

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Furin-cleavage site mutation

   Promoter

   CMV

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. MSCVhyg-HA-FLAG_Ubc9_WT

   Plasmid

   #164936

   Purpose

   Expresses FLAG & HA tagged human uman Ubiquitin Conjugating Enzyme 9 (UBC9) wild type cDNA

   Depositor

   Ji Luo

   Article

   Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

   Insert

   human UBC9 (UBE2I Human)
   

   Use

   Retroviral

   Tags

   FLAG & HA tags

   Promoter

   PGK promoter

   Available Since

   May 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pUC19-U6-FnCas12a crRNA-BsmBI cassette (BPK4446)

   Plasmid

   #114087

   Purpose

   U6 promoter crRNA entry vector used for all FnCas12a crRNAs (clone spacer oligos into BsmBI cassette)

   Depositor

   Keith Joung, Benjamin Kleinstiver

   Article

   Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

   Insert

   entry vector for FnCas12a crRNAs
   

   Expression

   Mammalian

   Available Since

   Dec. 11, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Lrrc4-Fc-His

   Plasmid

   #72084

   Purpose

   Expresses the extracellular region of the LRRC4 protein, C-terminally fused to the Fc region of human IgG1 + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Lrrc4 (Lrrc4 Mouse)
   

   Tags

   Fc-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 12, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pGL3-noSV40-humanEC1.45

   Plasmid

   #173946

   Purpose

   Firefly luciferase enhancer reporter plasmid.

   Depositor

   Joanna Wysocka

   Article

   Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

   Insert

   SOX9 enhancer cluster EC1.45
   

   Use

   Luciferase

   Mutation

   None

   Promoter

   SV40 promoter

   Available Since

   Sept. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRetroX-mCRT-GPI-IRES-DsRed

   Plasmid

   #223711

   Purpose

   Mammalian expression of mouse CRT-GPI

   Depositor

   Judy Lieberman

   Article

   Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

   Insert

   Calreticulin-GPI (Calr Mouse)
   

   Use

   Retroviral

   Expression

   Mammalian

   Available Since

   Sept. 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. lentiCRISPR - EGFP sgRNA 6

    Plasmid

    #51765

    Purpose

    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.

    Depositor

    Feng Zhang

    Article

    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:

    Inserts

    Cas9
    Puromycin resistance
    EGFP sgRNA 6
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    EFS and U6

    Available Since

    March 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. EF1a_MYC deltaNLS_P2A_Hygro_Barcode

    Plasmid

    #120506

    Purpose

    Barcoded lentiviral vector to express c-MYC deltaNLS in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    MYC H84NLS (MYC Human)
    

    Use

    Lentiviral

    Mutation

    Deletion of nuclear localization signal sequence

    Promoter

    EF1a

    Available Since

    Oct. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. p-miR-Report + WT miR-181 BS in GATA6 3UTR

    Plasmid

    #22794

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Wild-type miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

13. pCMV-CRT-Fc FL

    Plasmid

    #221853

    Purpose

    Express human Calreticulin (full-length) with C-terminal human IgG1Fc tag in mammalian cell.

    Depositor

    Judy Lieberman

    Article

    Sen Santara et al Nature. 2023 Apr;616(7956):348-356. doi:

    Insert

    calreticulin (CALR Human)
    

    Tags

    Human IgG1Fc tag

    Expression

    Mammalian

    Available Since

    July 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pExp-RBD-CHis

    Plasmid

    #195002

    Purpose

    Production of SARS-CoV2 receptor binding domain in E. coli with C-terminal Avi-tag

    Depositor

    Marko Hyvönen

    Article

    Fischer et al bioRxiv 2023.01.10.523494

    Insert

    RBD (S SARS-CoV-2)
    

    Tags

    His8

    Expression

    Bacterial

    Promoter

    T7lac

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. GLI1_pLENTI-CAG-IRES-GFP

    Plasmid

    #176992

    Purpose

    Mammalian lentiviral expression vector encoding GLI1

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Use

    Lentiviral

    Promoter

    CAG

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. p-miR-Report + MT miR-181 BS in GATA6 3UTR

    Plasmid

    #22795

    Depositor

    Xin Wang

    Article

    Ji et al Hepatology. 2009 Aug . 50(2):472-80.

    Insert

    Mutant miR-181 Binding Site in GATA binding protein 6 3 Untranslated Region (GATA6 Human)
    

    Use

    Luciferase

    Available Since

    March 24, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pGL4.23 A82V 2014 EBOV Delta-Mucin-Like-Domain

    Plasmid

    #86022

    Purpose

    Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

    Depositor

    Jeremy Luban, Pardis Sabeti

    Article

    Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

    Insert

    Makona Ebola Virus Glycoprotein
    

    Expression

    Mammalian

    Mutation

    Lacks mucin-like domain; Changed alanine 82 to va…

    Promoter

    CMV IE

    Available Since

    May 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG-hFnCas12a-NLS(nucleoplasmin)-3xHA (AAS1472)

    Plasmid

    #114089

    Purpose

    CAG promoter expression plasmid for human codon optimized FnCas12a nuclease with C-terminal NLS and HA tag

    Depositor

    Keith Joung, Benjamin Kleinstiver

    Article

    Kleinstiver et al Nat Biotechnol. 2019 Feb 11. pii: 10.103

    Insert

    human codon optimized FnCas12a
    

    Tags

    NLS(nucleoplasmin) and 3xHA

    Expression

    Mammalian

    Available Since

    Dec. 11, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. GLI1_pcDNA6.2/EmGFP-Bsd

    Plasmid

    #176968

    Purpose

    Mammalian expression vector encoding GLI1 and EmGFP-Bsd

    Depositor

    Roger Reeves

    Article

    Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

    Insert

    GLI1 (GLI1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    April 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCC_03 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-xCas9-NLS-2A-Puro-WPRE

    Plasmid

    #139088

    Purpose

    Expresses human codon-optimized xCas9 3.7 nuclease in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a unique barcode downstream of sgRNA cassette.

    Depositor

    Neville Sanjana

    Article

    Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

    Insert

    xCas9 3.7
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Mutation

    A262T, R324L,S409I, E480K, E543D, M694I, E1219V

    Available Since

    March 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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