We narrowed to 9,307 results for: try

1. pGTM_COV2_NSP5_C145A_001_SUMO

   Plasmid

   #192349

   Purpose

   Expresses SARS-CoV-2 3CLpro with an active site mutation (C154A) for the expression of the mature protease in an inactive form

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   SARS-COV-2 Mpro-C145A (NEWENTRY SARS-CoV-2)
   

   Use

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Mutation

   Cysteine 145 of the active site is substituted wi…

   Promoter

   T7 lac

   Available since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. SynNotch TMD/Gal4-VP64 ICD (MTK 3b) (pAN1031)

   Plasmid

   #194222

   Purpose

   Mammalian Toolkit part 3b encoding SynNotch and GAL4-VP64 to be used in designing modular receptors

   Depositor

   Hana El-Samad

   Article

   Weinberg et al bioRxiv 2021.04.20.440678

   Insert

   SynNotch-GAL4 DBD-VP64 AD
   

   Use

   Synthetic Biology

   Tags

   Expression

   Mutation

   Promoter

   None

   Available since

   Sept. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENTR-N90-beta-catenin

   Plasmid

   #31787

   Depositor

   Xin Chen

   Article

   Tward et al Proc Natl Acad Sci U S A. 2007 Sep 11;10

   Insert

   beta-catenin (CTNNB1 Human)
   

   Use

   Entry vector

   Tags

   Myc

   Expression

   Mutation

   Deletion of aa 1-90- Constitutively Active

   Promoter

   Available since

   Oct. 21, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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4. AAV2/9-CW3SL-mCherry-DrTrkB

   Plasmid

   #184002

   Purpose

   AAV production for expression of optically controlled eDrTrkB with N-terminal fusion of mCherry

   Depositor

   Vladislav Verkhusha, Dmitry Gerashchenko

   Article

   Leopold et al Nat Methods. 2022 Jul;19(7):871-880. doi

   Insert

   eDrTrkB (NTRK2 Human)
   

   Use

   AAV

   Tags

   Ig kappa secretory signal, mCherry, Deinococcus r…

   Expression

   Mutation

   Promoter

   Available since

   Dec. 8, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. dpYPQ230-D156R-CBE1

   Plasmid

   #195367

   Purpose

   dLbCas12a-D156R based cytosine base editor, hA3A/Y130F fused to the N terminal of dLbCas12a-D156R with the linker of XTEN with 3xFLAG

   Depositor

   Yiping Qi

   Article

   Cheng et al Plant Commun. 2023 Apr 13:100601. doi: 1

   Insert

   hA3A/Y130F-XTEN with 3xFLAG-dLbCas12a-D156R-UGI
   

   Use

   CRISPR; Gateway compatible ha3a/y130f-xten plus 3…

   Tags

   3xflag

   Expression

   Plant

   Mutation

   Promoter

   ZmUBI1

   Available since

   May 17, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p5E-hs_ICAM_full (JDW 1047)

   Plasmid

   #224479

   Purpose

   Gateway compatible 5' entry clone containing the Human ICAM2 promoter

   Depositor

   Joshua Wythe

   Article

   Gillespie et al bioRxiv [Preprint]. 2024 Jul 13:2024.07.

   Insert

   ICAM2 promoter
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. meffRed chromoprotein

   Plasmid

   #117836

   Purpose

   BioBrick pSB1C3 plasmid that constitutively overexpresses meffRed chromoprotein in E. coli

   Depositor

   Anthony Forster

   Article

   Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

   Insert

   promoter, RBS, meffRed
   

   Use

   Synthetic Biology; Escherichia coli

   Tags

   Expression

   Mutation

   BioBrick sites removed

   Promoter

   Available since

   Aug. 1, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pV1393

   Plasmid

   #111430

   Purpose

   CaCas9/gRNA Solo entry plasmid for cloning guides - contains stuffer with BsmBI sites - inserts at Neut5L - Recyclable for serial mutagenesis (Sap2-FLP)

   Depositor

   Gerald Fink

   Article

   Vyas et al mSphere. 2018 Apr 25;3(2). pii: 3/2/e001

   Insert

   CaCas9/sgRNA-BsmBI stuffer
   

   Use

   CRISPR

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Aug. 29, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. p2T-CAG-KKHSaCas9-BlastR

   Plasmid

   #107189

   Purpose

   Confers constitutive expression of KKH SaCas9.

   Depositor

   Richard Sherwood

   Article

   Shen et al Nature. 2018 Nov;563(7733):646-651. doi:

   Insert

   KKH SaCas9 (NEWENTRY )
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   E782K/N968K/R1015H

   Promoter

   Chicken ß-Actin

   Available since

   Dec. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. DT049A

    Plasmid

    #159410

    Purpose

    Constitutive murine mature BDNF expression, controlled under E.coli tet promoter

    Depositor

    Sheref Mansy

    Article

    Toparlak et al Sci Adv. 2020 Sep 18;6(38). pii: 6/38/ea

    Insert

    pr.tet-BDNF (Bdnf, Mouse) (Bdnf Mouse, Synthetic)
    

    Use

    Synthetic Biology

    Tags

    FLAG

    Expression

    Bacterial

    Mutation

    Promoter

    tet promoter

    Available since

    March 9, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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