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  2. Rosella: A Fluorescent pH-Biosensor for Studying Autophagy

    Type
    Blog Post
    ...radioactivity in the culture supernatant (this is indicative of protein degradation). To control for protein...studying autophagy. This method relies on the identification of autophagic structures based on morphology...

  4. All in a Twist: dsRNA

    Type
    Blog Post
    ...generate dsRNA as part of their infection and/or replication process within the host. dsRNA viruses are inherently...generate dsRNA intermediates as part of the viral replication process. dsDNA viruses yield dsRNA through a ...

  5. Transferable Skills Guide: Problem Solving

    Type
    Blog Post
    ...might even be expected to write up a report to communicate your findings to your colleagues. So perhaps ...about the issue, assessed potential protocol modifications, and ultimately decided on the changes to implement...

  6. What's New in CRISPR - November 2021

    Type
    Blog Post
    ...AsCas12f1. They found that this system, without any modifications, is effective in genome editing in both bacteria...therapeutically relevant gene targets and expand the application to the possible treatment of genetic disease....

  8. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    ... example, I will often see a whole bunch of notifications “a postdoc from this Uni viewed your profile...look straight at the camera. Pro tip: Turn off notifications to your network of profile changes. If you are...

  9. Developing Lab Management Software for Biology

    Type
    Blog Post
    ...for a scaleable lab. When you need to track the location, quality, growth, and legal status of thousands...stab culture Ship the stab culture to the proper location To keep ourselves from mixing up samples and ...

  10. Gendered Innovations: Why Does Sex of the Cell Matter?

    Type
    Blog Post
    .... To remedy this, Shah et al. provide a table indicating the sex of the most commonly used cell lines ... work to be done! Researchers need to learn sophisticated methods of sex and gender analysis. Universities...

  12. Working with Nuclear Receptors

    Type
    Blog Post
    ...murine members. NRs permit the integration and communication of such signals between central and peripheral...macrophage activation. Our mass spectrometry-affinity purification proteomic profiling of coregulators in HeLa ...

  14. Lab Automation at Addgene

    Type
    Blog Post
    ...efficient. Here, I’ll discuss transformation, DNA purification, and filling vials with agar. I’ll also provide...greater than 95% success rate! Automating DNA purification We're developing ways to prep large quantities...

  15. High Complexity Golden Gate Assembly with NEB’s DAD

    Type
    Blog Post
    ...assemblies are possible, the two sets of data together indicate that 35-pieces is a reasonable upper limit for...Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly. ACS Synthetic Biology. https...

  16. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    ...combined with. Combining a PSAM with the IPD of a cation channel, such as the serotonin 3 receptor, will...chemogenetics for research and potential clinical applications. Science 6436: eaav5282. https://science.sciencemag.org...

  17. How to Prepare for an Industry Interview

    Type
    Blog Post
    ...accomplishments and demonstrate your science communication skills. Know your audience. Connect your prior...codes for interviews. You can also ask HR for clarification on the dress code for the interview. Greet each...

  19. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type
    Blog Post
    ...the most common type of competitive ELISA is a modification of the sandwich ELISA as described here, the...ELISA, the only difference being the additional amplification step, which helps to visualize low-abundance...
Showing: 821 - 840 of 1022 results