...symptoms of a negotiation, especially when money is involved. However, it doesn’t have to be this way, if you...financial values. All too often, we see early investigators making unnecessary short term sacrifices that...their goals, rather than focusing on long term investments. For example, purchasing “used” equipment can...a win-win decision An institute/department is investing in you, and is counting on your research success...solutions to reach your goals. For example, a junior investigator turned down a faculty position because none ...opinions on creating and negotiating budgets and invite you to leave a comment below Happy Sciencing, ...

...expressing receptors Invasiveness Mice require permanent intracranial implant Noninvasive Controlling Stimulation...Targeted manipulation Controlling stimulation Invasiveness  Choosing the best set of tools therefore depends...precise and the offset of stimulation is gradual. Invasiveness Both optogenetic and chemogenetic tools need...Chemogenetics poses the advantage of being less invasive and more flexible as it relies on designer ligands...

...also present hurdles for the team of researchers involved. Communication is a major challenge when researchers...Set and manage expectations When the researchers involved in a project are scattered across the globe, it...labs. This is an opportunity for the researchers involved to learn techniques from each other, access specialized...to specialized microscopes and allowed me to investigate my protein of interest in a French clinical cohort...immediately discuss the feedback with everyone involved can speed up the future directions of the project...

... ability to send invitations. Finally, if you don’t know clearly why you are inviting someone to connect... box, it will send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn phone... app either as this also results in a generic invitation. 3) Connect with people you have really talked... with this and as long as you personalize the invitation with your intention for connecting, this is probably...

...strategies for CRISPR therapies. Systemic therapies involve CRISPR-Cas delivery directly to the patient. Patient-derived...returned to the patient. Finally, allogenic therapies involve editing healthy donor-derived cells and delivering... treatment strategies for these disorders have involved turning on fetal hemoglobin to compensate for ...returning them to the patient. The first human trial involving CRISPR Cas9 for these diseases was sponsored by...evolving, and many of the challenges with therapies involve patient safety, effectiveness, and delivery. Patient...

...separate elements of the R-mAb expression plasmid involved in coexpression of light (green) and heavy (blue...

...damage public trust in scientists, experiments involving potential gene drives should be conducted with...strategies are available? Molecular confinement involves building gene drives that can spread through populations... been tested in yeast5. Ecological confinement involves performing experiments in a geographic area where...relevant mosquito species. Reproductive confinement involves working with laboratory organisms that can't reproduce...publications. Thank you to our guest blogger! An inventor of technologies that harness evolution, Kevin ...

...in a range of biological studies, including investigations into environmental pollutants and health, embryo...developmental biology that arose from zebrafish-based investigations. Genetic resources for zebrafish A common ...about the genome are powerful resources for investigating any biological process. High quality genome ...are particularly well-suited for neuroscience investigations. For example, neuronal activity can be tracked... and labor-intensive maintenance compared to invertebrate model systems, and their resistance (so far)...

...factors to repress the expression of genes primarily involved in development. These genes are known to play ... a comic book his daughter was reading. SHH is involved in the hedgehog signalling pathway, which is a...resemble spaghetti. We have two plasmids in our inventory from the lab of Eric Wieschaus that express sqh...Azplazu and Frasch, 1993). Tinman is specifically involved in the formation of the heart amongst other things...Transcriptional activation in Drosophila spermatogenesis involves the mutually dependent function of aly and a novel...

...library and used this library to screen for genes involved in West Nile Virus (WNV) induced cell death. The...for their gene of interest. Although the parts involved in altering gene expression are relatively well...can be quite arduous. The cloning process alone involves much literature searching, DNA synthesis, and ... Collection 2.0 Ras proteins are small GTPases involved in cell signaling pathways that control many cellular...AID technologies provides a clever means of investigating hard-to-study genes in hard-to-study cell types...screening is an efficient approach to identify genes involved in drug-resistance. Remarkably, this method also... gondii fitness. Sixteen of these genes were investigated for functions during infection of human cells...

...team also investigates bacteria and extremophiles for biotechnology and has been directly involved in supervising... through Open Access. Chapter 7/Outro: Tom’s Involvement with iGEM and Addgene  Many thanks to our guest... He returned to academic research in 2005 to investigate synthetic biology at Boston University, working...

... website, write a blog Pursue speaking invitations by inviting others (be on a speaker committee) Follow...undergrads or less experienced grads Manage a project involving multiple scientists, create and stick to timelines...science careers is that a team is almost always involved. Demonstrating your ability to work well with ...

...efficiency of the method. One observation was an inverse relationship between deletion size and frequency...outcome to deletions following paired DSBs is inversions [3]. These rearranged segments may also be informative...nondeleted allele is likely to bear an indel or inversion mutation [3]. In the CRISPR era, the genomic landscape...Bauer is a pediatric hematologist and principal investigator at Boston Children’s Hospital and Harvard Medical...

.... Widening your search The first step towards inviting serendipity into your research lies in expanding...approaches One approach to achieve this goal could involve participating in interdisciplinary seminars, such...Additionally, journaling in the morning has proven invaluable for me, allowing me to pinpoint areas of my career...openness to unexpected possibilities, you not only invite serendipity into your research but also cultivate...

...various cas genes not only to store a record of invading phages but to also destroy the phage upon re-exposure... the foreign DNA, a cut is made to destroy the invader. Wikipedia, accessed 25 November 2013. Author...systems provides powerful lines of defense against invading phages. Rising to the challenge - Evolution of...research. Do you have a cool idea or application that involves CRISPR/Cas technology? Let us know.  References...

...2016 Addgene’s research team has been actively investigating ways to improve viral vector production. Whether...Whether redesigning viral packaging plasmids, investigating different viral production strategies or developing...method to improve AAV yields. Although we only investigated 3 serotypes it’s possible that this approach...less time and resources resupplying our current inventory and more time providing new materials to the research...

...the superheroes win in the end. But we’re still invested in seeing how they do it.    Finding Conflict...even when we know the ending, we can still get invested in the journey? The same thing applies for science...communication, use conflict to get your audience invested in the more complex story, and then build on that...gives your audience a chance to get curious and invested in the research itself - and isn’t that sense ...

...researchers, such as postdocs and principal investigators. They also come from many different scientific...scientific research. We continue to support those involved in COVID-19 research, but our new platform Crowdfight...we do in our Frequently Asked Questions. We also invite you to participate in the 1st Crowdfight Symposium...is a postdoctoral researcher at Instituto de Investigación Biomédica de Salamanca (IBSAL) and one of the...

...roles involves on a dad-to-day basis. Often, it is hard to know what a job title actually involves, other...These skills show employers that you can fully investigate and solve a problem or complete a project, overcoming...passionate and capable. For example if the job involves traveling, showing that you are a highly organised...

...to get involved with DIY bio If you’re not in the Boston area but are inspired to get involved, check ...personal projects in mind. Thus, to encourage more involvement and provide all our members the opportunity to...previous city-wide genomic studies and our study will involve getting the community to collect various soil samples...