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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 359 results for: Nov

Showing: 121 - 140 of 359 results

  1. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    ...Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...of EvolvR The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant...spectinomycin, rpsE. These results not only uncovered novel resistance genotypes, but also provide a better ...

  2. 3 Challenges in Plant Synthetic Biology

    Type
    Blog Post
    ...; synthetic biology is required to achieve this novel and unique phenotype. It’s an unexpected use of ...creating new forums and opportunities for open innovation in plant synthetic biology. In our case, we selected...off patent, or not patented, and building our own novel pieces where applicable. We will also be working...

  3. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    ...one, is a sterling example of collaboration and innovation between legal and scientific fields.” Thanks ... every day for their dedication, flexibility, innovative thinking, and openness to team work.   Company...positions. We have an awesome talent pipeline. Turnover at Addgene is about half of what is standard in...

  4. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    ...Research Strategy highlights i) the significance and innovation of the project and ii) how you, the expert, will...writing a proposal to test hypotheses and develop novel technologies! If data from a publication or preliminary...funding. Convince the readers why and how your innovation will address a variety of challenges and impact...

  5. Viral Vectors 101: Virus Safety

    Type
    Blog Post
    ... in the lab are gamma retrovirus, lentivirus, adenovirus, and adeno-associated virus (AAV). The least ...is usually classified as a BSL-1 safety level. Adenovirus is similar to AAV in that it does not integrate...integrate into the host’s genome. However, some adenovirus strains are known to cause respiratory diseases ...

  6. A Look at Addgene's QC Process

    Type
    Blog Post
    ...assembled into a circularized plasmid sequence de novo (without the use of a reference sequence) using ...the plasmid, QC scientists will first align the de novo NGS result to the deposited sequence(s) in SnapGene...if one was provided. If no reference is given, de novo assembly in Geneious can still provide additional...

  7. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    ...replicate itself and depends on helper viruses like adenovirus (Ad) or herpes simplex virus to facilitate efficient...a “helper-free” system, in which the required adenoviral helper genes are supplied on two different packaging..., J. (1999). Titration of AAV-2 particles via a novel capsid ELISA: Packaging of genomes can limit production...

  8. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    ...expressing your gene, especially if you are studying a novel gene, is not always a straightforward process. Rather... of interest, scientists have instead developed novel methods to co-express your gene along with a reporter...more time developing the clever experiments and innovative solutions necessary for advancing the field. ...

  10. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...Aleksandra Radenovic 114194 pc DNA FRT TO- APPSwed/Ind APP CMV Alzheimer's Aleksandra Radenovic 114410 AICSDP...iLID CBA ALS Anna Akhmanova 240425 HA-KIF5A-FRB KIF5A HA, FRB CMV ALS Anna Akhmanova 240582 pET21a asyn...

  11. Rett Syndrome: A History of Research and Therapeutic Outlooks

    Type
    Blog Post
    ...co-founded by Kaspar, acquired by Novartis and rebranded to Novartis Gene Therapies, and Taysha Gene Therapies... in tackling the root cause of Rett Syndrome in novel ways.  Addgene’s MECP2 Plasmid Collection and Resource...

  12. Deciphering the Mysteries of Behavior with Viral Vectors

    Type
    Blog Post
    ...locomotor sensitization and might open new ways for a novel therapeutic approach against drug addiction. Anxiety...illuminated ones (risky). They also prefer to explore novel environments by initially moving along a wall (protected...bright place, driven by their motivation to explore novel environments, rather than being inhibited by fear...

  14. Healthcare Consulting: A Door to the Business of Life Sciences

    Type
    Blog Post
    ... publish on the health and economic benefits of novel drugs. Commercial Affairs teams make up a large ...for cancer with chemotherapies, or developing innovative mobile phone applications and services that help...the more challenging parts of the job? With fast turnover of projects, pressure to maintain consistently...

  15. Quick Guide to Near-Infrared Fluorescent Proteins

    Type
    Blog Post
    ...developed NIR split probes (Tchekanda et al., 2014, Filonov et al., 2013). FRET biosensors were developed based... interactions. Nat Methods 11, 641-644 (2014). Filonov, G.S. & Verkhusha, V.V. A near-infrared BiFC reporter... vivo imaging. Nat Methods 10, 751-754 (2013). Filonov, G.S. et al. Bright and stable near-infrared fluorescent...

  16. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    ...inhibited. Recently, we at the Institute for Protein Innovation (IPI), a Boston-based, nonprofit protein research...recombinant antibodies (rAbs) either produced de novo using yeast display technology or produced from ...a science writer at the Institute for Protein Innovation. She writes about all things IPI, from the scientists...

  17. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    ...for inhibition of AcrB multidrug efflux pump by novel and powerful pyranopyridine derivatives." Proceedings...Academy of Sciences 113.13 (2016): 3509-3514. Tikhonova, Elena B., et al. "A transcription blocker isolated...metalloproteinase: López-Pelegrín, Mar, et al. "A novel mechanism of latency in matrix metalloproteinases...

  18. The Pleiades Promoter Project

    Type
    Collection
    ... Imanian, B., Jiang, S., Khorasan-zadeh, S., Komljenovic, I., Laprise, S., Liao, N. Y., Lim, J. S., Lithwick...

  20. Using AAV for Neuronal Tracing

    Type
    Blog Post
    ...vesicular stomatitis virus (VSV; Beier et al., 2011), adenovirus, and adeno-associated virus (AAV), have been ...the California Institute of Technology generated novel AAVs (Chan et al., 2017) that express one of a few...type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits. Nat Neurosci 17(2), 269...
Showing: 121 - 140 of 359 results