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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 4 results for: CBR luciferase

Showing: 1 - 4 of 4 results

  1. Fluorescent Proteins 101: Luciferases

    Type
    Blog Post
    ...expressing luciferase Luciferase reporter assays for monitoring gene regulation Since firefly luciferase was ...Well-established Natural and engineered luciferases Although firefly luciferase was the first bioluminescent reporter...have engineered a variety of improved luciferases. NanoLuc® Luciferase is the best known: it’s derived from...Oplophorus gracilirostris) luciferase and engineered to be extra bright. Other luciferases have been modified ...mammalian systems. Our luciferase collection page has a detailed list of luciferase options. Table 2: Properties...element is cloned upstream of the luciferase gene, and expression of luciferase is measured via the light produced... single protein. Considerations for luciferase assays Luciferases can only produce light in the presence...

  2. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    ...nonfluorescent (Halo, SNAP) tags, a selectable marker (floxed Cbr-unc-119) for easy screening of the insertion event...donor plasmids for protein tagging, including luciferase (NanoLuc), fluorescent proteins (TagGFP2, TagBFP...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...Protein A-Renilla luciferase and the “prey” proteins are tagged with Firefly luciferase. The bait and prey... sequences, including fluorescent tags, a floxed Cbr-unc-119 selectable marker, and “connectors” that ...immunoprecipitated from the cells using the Protein A tag. The luciferase activities can then be measured and normalized... assess interactions. Higher relative firefly luciferase activity in the Co-IP compared of a control Co-IP...

  4. Luciferase Plasmid Collection

    Type
    Collection
    ... of luciferase found in Addgene's collection include: Firefly luciferase (Fluc) Renilla luciferase (Rluc...respectively), Cypridina luciferase , and Luciola luciferase . Each type of luciferase has advantages and disadvantages...CMV Dual secreted luciferase reporter (EF1α-Gaussia luciferase, CMV-Cypridina luciferase). Feng Zhang Return...Other luciferases in our collection include the red and green Click-beetle luciferases ( CBRluc and CBGluc... Plasmid Collections Luciferase Plasmids Luciferase Plasmid Collection Empty Backbones Expression...Fluorescent Proteins Blog: Luciferase 101 Blog: Technologies Enabled by NanoLuc® Luciferase Blog: Luminescent ...Gaussia luciferase (Gluc) NanoLuc® (Nluc) The mammalian codon-optimized version of firefly luciferase is often...
Showing: 1 - 4 of 4 results