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Showing: 1 - 13 of 13 results
  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Updated
    Nov. 19, 2019, 3:08 p.m.
    ...protein expression Under the direction of Sabine Suppmann, the Recombinant Protein Production group at Max-Planck...MitoTimer proteins are derived from the Timer protein - a mutant of the fluorescent protein dsRed that...established a dual-color RNAi “sensor” mouse strain in which Cre expression causes the induction of both DsRed...Up to eight entry vectors can be used for each Multisite Gateway compatible destination vector for a maximum...and clonogenic neural stem cells concomitant with activated DsRed2 expression....Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre alleles showed efficient GFP knockdown in eyes...
  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Updated
    Oct. 27, 2019, 6:17 p.m.
    ...Erich Wanker’s group at the Max-Delbrueck Center for Molecular Medicine has recently developed a new...The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while SEP fluoresces at cellular...cell, but turns more red in lower (e.g. vacuolar) pH environments that inactivate SEP and leave only DsRed...after the brightly-coloured Australian parrot, the dual colour-emission biosensor Rosella consists of DsRed...cells, targets 13,501 fly genes with at least three independent gRNAs, each chosen strategically to maximize...
  3. Cre-lox system

    Type
    Collection
    ...Cre activates your gene fused to eGFP, removes dsRed....Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid Mammalian Green 22799 Ai9 Rosa26...and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP Switches...from DsRed to eGFP expression upon the presence of Cre Lentiviral van Rheenen 28304 pAAV-FLEX-GFP...Plasmid 15037 also contains IRES-EGFP Mammalian Costantini 32702 pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE...ON or OFF following Cre recombination to maximize label diversity Mammalian Megason 13769 pCALNL-DsRed...pCAG-loxPSTOPloxP-ZsGreen Cre dependent ZsGreen expression Mammalian Pelczar 62732 Cre Reporter DsRED...which reporters are initially OFF and then probabilistically ON or OFF following Cre recombination to maximize...label diversity Mammalian Megason 13769 pCALNL-DsRed Cre dependent DsRed expression Mammalian Cepko...13770 pCALNL-GFP Cre dependent GFP expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed...
  4. CRISPR References and Information

    Type
    Collection
    ...(pSIR-DsRed-Express2), human CD2 (pSIR-hCD2) PDF (105.8 KB) Fujii...Retroviral vectors: neomycin (pSIR-neo), GFP (pSIR-GFP), DsRed...pAC2, pAC152, pAC153, pAC154; pmax..., rice (Oryza sativa), tomato, corn, monkey (macaca mulatta).Cas-Designer searches for targets that maximize...dsDNA donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED...
  5. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ...Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR FUGW...(unpublished) expression of FnCas9 and DsRed.../Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max...doi: 10.1080/21645698.2015.1106063. 2x35S promoting Glycing Max...pCDNA3.3 CAS9 IRES-DsredFnCas9 IRES-Dsred...pX458-DsRed2DsRed2...pLV-U6-gRNA-UbC-DsRed-P2A-BsrDsRed-Express2...Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2 pX458...IRES-Dsred (Synthetic) Laur...
  6. Adeno-associated virus (AAV) Plasmids

    Type
    Collection
    ...pTCAV-FLEx(loxP)-FlpOFlpOMammalian ExpressionpTCAV Luo pAAV-hsyn-flex-dsRed-shvgatAAV-hsyn-flexed-dsRed-miR30...shRNAactin prom-dsRed-scramble shRNA (Gallus gallus)Mammalian Expression, AAV, RNAipUC...Wisden AAV-actin prom-dsRed-adra2a.1227.shRNAdsRed and shRNA to knock down the mouse adra2...synapsin promoter-flex switch-dsRed-shscramble (Homo sapiens)Mammalian Expression, AAV, RNAi, Cre/LoxpUC...Expression, AAVpAAV Callaway pAAV-fSST-RFPfugu somatostatin promoter driving DsRed...Expression, Mouse Targeting, AAV, RNAi, Cre/LoxpUC Wisden AAV-actin prom-dsRed-scramble...protein (Other)Mammalian Expression, AAVpAAV Oertner pAAV-hysn-flex-dsRed-shscramblehuman...Wisden AAV-actin prom-dsRed-adra2a.1227.shRNAdsRed and shRNA to knock down the mouse adra2...pAAV-CAG-dLight1.2dLight1.2 (Synthetic)AAVpAAV-CAGFlag tag Tian pAAV-hSynapsin1-axon-GCaMP6maxon-GCaMP6m...AAVpAAV Callaway pAAV-hADM-RFPhuman adrenomedullin promoter driving DsRedExpress...pAAV-hNR2F2-RFPhuman nuclear receptor subfamily 2 group F member 2 promoter driving DsRedExpress...
  7. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...specifically optimized for yeast Lindquist Lab Vectors - Gateway cloning; includes tagging with ECFP, EYFP, DsRed...563 582 24 Tetramer pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian Expression DsRed2-C1...Maturation is the time for fluorescence to obtain half-maximal value....Backbones for Fluorescent Protein Fusions (Organized by Color) For each table: Excitation and Emission maximum...coefficient and quantum yield divided by 1000. pKa is the pH at which fluorescence intensity is 50% of its maximum...- Mammalian Expression DsRed2-pBAD - Bacterial Expression mApple 568 592 37 6.5 30 min Monomer...C1 - Mammalian Expression eqFP611 559 611 35 Tetramer eqFP611-N1 - Mammalian Expression DsRed2...
  8. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ...Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR FUGW...(unpublished) expression of FnCas9 and DsRed...pCDNA3.3 CAS9 IRES-DsredFnCas9 IRES-Dsred...pLV-U6-gRNA-UbC-DsRed-P2A-BsrDsRed-Express2...pX458-DsRed2DsRed2...pLV-U6-gRNA-UbC-DsRed-P2A-BsrDsRed-Express2...Cloning backbone for sgRNA, co-expresses human optimized S. pyogenes Cas9 and DsRed2 pX458...IRES-Dsred (Synthetic) Laur...pCDNA3.3 CAS9 IRES-DsredFnCas9...
  9. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...RFPec2)Bacterial Expression, Synthetic Biology Keasling Optimization of DsRed...pDsRed (GB0100)Provides the CDS of DsRed as a level 0 GoldenBraid partDsRed coding region (Other)Synthetic...pBBRBB-Ppuf843-1200-DsRedExpressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides...pEGB 35S:DsRed:Tnos (GB0361)Transcriptional unit for DsRed plant expression driven by the 35S promoterDsRed...(unpublished) pEF 4r-DsRed...pBBRBB-Ppuf1-1200-DsRedExpressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroidesDsRedSynthetic...pDsRed (GB0100)Provides the CDS of DsRed as a level 0 GoldenBraid partDsRed coding region (Other)Synthetic...pEGB 35S:DsRed:Tnos (GB0361)Transcriptional unit for DsRed plant expression driven by the 35S promoterDsRed...pBBRBB-Ppuf1-1200-DsRedExpressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroidesDsRedSynthetic...pBBRBB-Ppuf843-1200-DsRedExpressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides...pJM100: no promoter RFP on pCM66TpCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)RFP...pJM104: pTrc RFP on pCM66TpCM66T with promoter pTrc driving RFP expression (maxRFP...pJM105: pTac RFP on pCM66TpCM66T with promoter pTac driving RFP expression (maxRFP...
  10. Plasmids for Stem Cell Research

    Type
    Collection
    ...pBRPyCAG-fmKlf4-DsRed-IpKlf4 (Mus musculus)Mammalian Expression, Cre/Lox Jaenisch...PB-XL-AncBE4maxAncBE4max_GFP (Synthetic)Mammalian Expression, CRISPR ; piggyBAC DNA transposon...
  11. Synthetic Biology - Bacterial

    Type
    Collection
    ...RFPec2)Bacterial Expression, Synthetic Biology Keasling Optimization of DsRed...pBBRBB-Ppuf843-1200-DsRedExpressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides...pBBRBB-Ppuf1-1200-DsRedExpressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroidesDsRedSynthetic...pBBRBB-Ppuf1-1200-DsRedExpressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroidesDsRedSynthetic...pBBRBB-Ppuf843-1200-DsRedExpressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides...pJM100: no promoter RFP on pCM66TpCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010)RFP...DsRedSynthetic Biology Schmidt-dannert BioBrick compatible vector system...pJM104: pTrc RFP on pCM66TpCM66T with promoter pTrc driving RFP expression (maxRFP...pJM105: pTac RFP on pCM66TpCM66T with promoter pTac driving RFP expression (maxRFP...
  12. CRISPR Plasmids - gRNAs

    Type
    Collection
    .../Cas9 and TALENs generate heritable mutations for genes involved in small RNA processing of Glycine max...CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pAdsRed2 (Homo sapiens)Mammalian Expression...CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pAdsRed2 (Homo sapiens)Mammalian Expression, Synthetic Biology...CMVp-dsRed2-Triplex-28-gRNA1-28-pAdsRed2 (Homo sapiens)Mammalian Expression, Synthetic Biology...Mammalian Expression, Lentiviral, CRISPR Root Optimized sgRNA design to maximize...
Showing: 1 - 13 of 13 results