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Showing: 1 - 14 of 14 results
  1. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    ...GFP derivatives, but are instead related to the dsRed protein isolated from Discosoma sp. Similar work...Articles Addgene's Fluorescent Protein Guide Note: A. Max Juchheim contributed to the writing of this article...
  2. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    ...the induction of both DsRed fluorescence and GFP shRNA. Mice carrying the R26DsRedR; CRUSH-GFP; and Nestin-Cre...Timer protein - a mutant of the fluorescent protein dsRed that changes irreversibly its color from green to...Suppmann, the Recombinant Protein Production group at Max-Planck Institute of Biochemistry has developed a ...Multisite Gateway compatible destination vector for a maximum of 24 elements assembled into a final transgenesis...clonogenic neural stem cells concomitant with activated DsRed2 expression. Brown et al., Genesis 2014 Jan;...
  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    ...dual colour-emission biosensor Rosella consists of DsRed connected to a pH-sensitive variant of GFP (SEP)...Figure 1). The key to the biosensor lies in pH: DsRed is relatively pH-insensitive, while SEP fluoresces...environments that inactivate SEP and leave only DsRed to fluoresce (1). With variants that can be targeted...interaction strengths. Erich Wanker’s group at the Max-Delbrueck Center for Molecular Medicine has recently...independent gRNAs, each chosen strategically to maximize the likelihood of creating a functional knock-...
  4. CRISPR Plasmids - Double-Strand Break (Cut)

    Type
    Collection
    ... pCW-Cas9 (#50661) pLV-U6-gRNA-UbC-DsRed-P2A-Bsr DsRed-Express2, Bsr hUbC Blasticidin Gersbach CRISPR-Cas9...construct pX330 pCDNA3.3 CAS9 IRES-Dsred FnCas9 IRES-Dsred (Synthetic) Laur Emory Custom Cloning Core...1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40 Rcd-1r-NLS-hSpCas9-T2A-GFP, Opie2-dsRed-SV40 (Drosophila melanogaster...Glycing Max codon optimized Cas9 with bar plant selection pMDC123 Cas9 MDC32 Glycine Max codon...pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP hsp70l promoter (Danio rerio), floxed DsRed cassette (Synthetic.... Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR FUGW pLV-U6-gRNA-UbC-eGFP-P2A-Bsr...Oskar Laur (unpublished) expression of FnCas9 and DsRed pCDNA3.3 CAS9 p1195-pssAAV.U1a.hNme2Cas9...
  5. CRISPR Plasmids - Plants

    Type
    Collection
    ...Glycing Max codon optimized Cas9 with bar plant selection pMDC123 Cas9 MDC32 Glycine Max codon...gRNA Shuttle pPZP Cas9 MDC123 Glycine Max codon optimized Cas9 (Other) Basta Stupar CRISPR/...10.1080/21645698.2015.1106063. 2x35S promoting Glycing Max codon optimized Cas9 with hygromycin plant selection...selection pMDC32 G10 Cas9 MDC123 Glycine Max codon optimized Cas9 (Other) Basta Stupar CRISPR/...10.1080/21645698.2015.1106063. G10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with ...genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnol J. 2017...
  6. Cre-lox system

    Type
    Collection
    ...recombination to maximize label diversity Mammalian Megason 13769 pCALNL-DsRed Cre dependent DsRed expression... pMSCV-loxp-dsRed-loxp-eGFP-Puro-WPRE Cre activates your gene fused to eGFP, removes dsRed. See article...Reporter DsRED and EGFP Cre recombinase reporter Lentiviral Geijsen 65726 pLV-CMV-LoxP-DsRed-LoxP-eGFP...expression Mammalian Cepko 8389 p212 pCMV-EGFP/RFP EGFP-dsRed gene switch plasmid Mammalian Green 22799 Ai9 Rosa26...pLV-CMV-LoxP-DsRed-LoxP-eGFP Switches from DsRed to eGFP expression upon the presence of Cre Lentiviral van Rheenen 28304 pAAV-FLEX-GFP...
  7. CRISPR References and Information

    Type
    Collection
    ...vectors: neomycin (pSIR-neo) , GFP (pSIR-GFP) , DsRed (pSIR-DsRed-Express2) , human CD2 (pSIR-hCD2) PDF (105.8...mulatta). Cas-Designer searches for targets that maximize knockout efficiency while having a a low probability...dual expression: pAC2 , pAC152 , pAC153 , pAC154 ; pmax dCas9 activator expression: pAC91 , pAC92 , pAC93...donor plasmid backbones pSL1180-HR-PUbECFP & pSL1180polyUBdsRED PDF (583.3 KB) Zhang gRNA cloning CRISPR ...
  8. CRISPR Plasmids - Zebrafish

    Type
    Collection
    ...pTol2 hsp70l loxP-DsRed-loxP-nCas9n-GFP hsp70l promoter (Danio rerio), floxed DsRed cassette (Synthetic... SpCas9 (Other) Bacterial Expression T7 Jinek Maximizing mutagenesis with solubilized CRISPR-Cas9 ribonucleoprotein...
  9. Fluorescent Protein Guide: Empty Backbones

    Type
    Collection
    ...Gateway cloning; includes tagging with ECFP, EYFP, DsRed, Cerulean Bacteria Gradia Lab Bacterial Vectors ...Mammalian Expression DsRed2 563 582 24 Tetramer pCAG-DsRed2 - Mammalian Expression DsRed2-N1 - Mammalian Expression... 50% of its maximum value. Maturation is the time for fluorescence to obtain half-maximal value. Structure... Expression DsRed2-C1 - Mammalian Expression DsRed2-pBAD - Bacterial Expression mApple 568 592 37 6.5 ...Color) For each table: Excitation and Emission maximum are listed in nm. Brightness is the product of ...
  10. Synthetic Biology - Bacterial

    Type
    Collection
    ...1200-DsRed Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroides DsRed Synthetic...1200-DsRed Expressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides DsRed Synthetic...Expression, Synthetic Biology Keasling Optimization of DsRed production in Escherichia coli: effect of ribosome...resistance (Synthetic) Synthetic Biology Melis Maximizing photosynthetic efficiency and culture productivity... pCM66T RFP control vector: no promoter driving maxRFP (BBa_E1010) RFP (BioBrick part number BBa_E1010...pCM66T with promoter pHps2 driving RFP expression (maxRFP = BBa_E1010) RFP (BioBrick part number BBa_E1010...shortened pHps2 promoter driving RFP expression (maxRFP = BBa_E1010) RFP (BioBrick part number BBa_E1010...
  11. Synthetic Biology - Browse Plasmids

    Type
    Collection
    ...1200-DsRed Expressed DsRed using the Ppuf1-1200 promoter segment, for use in R. sphaeroides DsRed Synthetic...1200-DsRed Expressed DsRed using the Ppuf843-1200 promoter segment, for use in R. sphaeroides DsRed Synthetic...3):1618-31. pDsRed (GB0100) Provides the CDS of DsRed as a level 0 GoldenBraid part DsRed coding region...vector Richart FQG plasmids (unpublished) pEF 4r-DsRed-3r DsRed Synthetic Biology ; plant Gateway vector Richart...Jul;162(3):1618-31. pEGB 35S:DsRed:Tnos (GB0361) Transcriptional unit for DsRed plant expression driven...Expression, Synthetic Biology Keasling Optimization of DsRed production in Escherichia coli: effect of ribosome...driven by the 35S promoter DsRed (Other) Plant Expression, Synthetic Biology Orzaez GoldenBraid 2.0: a comprehensive...
  12. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... pCW-Cas9 (#50661) pLV-U6-gRNA-UbC-DsRed-P2A-Bsr DsRed-Express2, Bsr hUbC Blasticidin Gersbach CRISPR-Cas9...construct pX330 pCDNA3.3 CAS9 IRES-Dsred FnCas9 IRES-Dsred (Synthetic) Laur Emory Custom Cloning Core.... Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR FUGW pLV-U6-gRNA-UbC-eGFP-P2A-Bsr...Oskar Laur (unpublished) expression of FnCas9 and DsRed pCDNA3.3 CAS9 p1195-pssAAV.U1a.hNme2Cas9...Expresses evoAPOBEC1-BE4max-NG in mammalian cells pBT195 pCMV-ABEmaxAW ABEmax(TadA E59A, TadA*...May. Expresses ABEmaxAW in mammalian cells CMV pCMV-ABEmax(TadA E59A) ABEmax(TadA E59A) (Homo...Expresses ABEmax(TadA E59A) in mammalian cells CMV pCMV-ABEmax(TadA E59A, TadA*E59A) ABEmax(TadA...
  13. Plasmids for Stem Cell Research

    Type
    Collection
    ...Acad Sci U S A. 2010 Jul 26. ():. pBRPyCAG-fmKlf4-DsRed-Ip Klf4 (Mus musculus) Mammalian Expression, Cre...doi: 10.1016/j.molcel.2019.06.045. PB-XL-AncBE4max AncBE4max_GFP (Synthetic) Mammalian Expression, CRISPR...
  14. CRISPR Plasmids - gRNAs

    Type
    Collection
    ...genes involved in small RNA processing of Glycine max and Medicago truncatula. Plant Biotechnol J. 2017...doi: 10.1128/mBio.01114-14. CMVp-dsRed2-Triplex-28-gRNA1-28-pA dsRed2 (Homo sapiens) Mammalian Expression...
Showing: 1 - 14 of 14 results