We narrowed to 6 results for: pCas

...pTargetF into the BL21(DE3)/pCas strain. In response, we modified the pCas/pTargetF system and updated...these CRISPR systems. The original pCas/pTargetF system Our original pCas/pTargetF system consists of one...the use of CRISPR editing systems in bacteria. Our pCas/pTargetF system has been one of the most popular...pEcCas/pEcgRNA system We speculated that the gRNA on pCas that is specific to the pMB1 replicon of pTargetF...transformants. Our speculation motivated us to update the pCas/pTargetF system.   Figure 1: The editing ...gRNA-pMB1 with PrhaB, 2) changed the replicon of pCas into a non-temperature-sensitive replicon, and 3...targeting the genomic site of interest. We compared the pCas/pTargetF system with the updated pEcCas/pEcgRNA ...

...iterative genome engineering (Jiang et al., 2015). pCas contains Cas9 and the phage recombination gene lambda... of editing takes two days, and the pTargetF and pCas plasmids can be subsequently cured from the bacteria...pEcCas/pEcgRNA system works similar to the original pCas/pTargetF but has many other advantages including...Chen J, Zhang Y, Jiang Y, Yang S (2021) A modified pCas/pTargetF system for CRISPR-Cas9-assisted genome ...

...As evidenced by all the CRISPR publications, press, and plasmids out there, it’s obvious that CRISPR ...

...Split-BioID combines a protein fragment complementation (PCA) technique with proximity labelling to validate binary...additional interacting proteins in one simple experiment. PCA is an in vivo technique where two proteins are fused...

...called a protein fragmentation complementation assay (PCA). In the Split-BioID method, each BirA* fragment ...