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We narrowed to 51 results for: rpe

Showing: 1 - 20 of 51 results

  1. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ...infecting cells of the retinal pigmented epithelium (RPE). AAVs, on the other hand, have broad tropism in...Luckily for patients, this gene can be targeted to RPE cells using a lentiviral vector and a clinical trial...after the cloning of the RPE65 gene, a naturally occurring animal model, the RPE65−/− dog, was characterized...of several disease-causing genes, including the RPE65 gene responsible for an autosomal recessive, severe...study showing that AAV-mediated delivery of the RPE65 gene restores vision in the dog model of LCA (Lancelot...Lancelot, one of the dogs who received the AAV-RPE65 treatment, famously visited Congress to help increase...

  2. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...Pancreas AAV8 Photoreceptor Cells AAV2, AAV5, AAV8 RPE (Retinal Pigment Epithelium) AAV1, AAV2, AAV4, AAV5...

  3. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...AAV7, AAV8, AAV9 Lung AAV4, AAV5, AAV6, AAV9 RPE (Retinal Pigment Epithelium) AAV1, AAV2, AAV4, AAV5...untreated (for review see Dietzschold et al., 2008). Herpes Simplex Virus (HSV) also migrates through neurons... Strick, P.L. (1989). Transneuronal transfer of herpes virus from peripheral nerves to cortex and brainstem...

  4. X-CHIME: Context Dependent Germline Knockout in Immune Cells

    Type
    Blog Post
    Published
    Jan. 16, 2024, 6:13 p.m.
    ..., developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo gene...Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested...Montero Llopis, P., Haining, W. N., Doench, J. G., & Sharpe, A. H. (2024). X-CHIME enables combinatorial, inducible..., Al Abosy, R., Freeman, G.J., Haining, W.N., & Sharpe, A.H. (2019). PTPN2 regulates the generation of..., Coxe, D. J., Doench, J. G., Haining, W. N., & Sharpe, A. H. (2019). A CRISPR-Cas9 delivery system for...

  5. Celebrating Outstanding CRISPR/Cas9 Achievements at the Dr. Paul Janssen Award Dinner

    Type
    Blog Post
    Published
    Sept. 15, 2014, 6:05 p.m.
    ...engineering, Dr. Jennifer Doudna and Dr. Emmanuelle Charpentier. I was joined by Addgene Scientist Matt Ferenc...and Joan Steitz. This award ceremony honored Charpentier and Doudna, who talked about their research leading...simpler 1 protein - 1 RNA system Drs. Dounda and Charpentier met at a 2011 conference and decided to collaborate...was a simpler 1 protein - 1 RNA system.  Dr. Charpentier advocated for the crucial role of basic research...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...against each other, you should be able to draw perpendicular lines that intersect your populations. If you...population needs to be shifted, consider your perpendicular lines. In this case, the population on the left...needs to shift towards the X axis to become a perpendicular vertical line.     In general, most spillover...She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More resources...

  7. Performing In Vivo CRISPR Screens Using the FITS Approach

    Type
    Blog Post
    Published
    May 2, 2024, 1:15 p.m.
    ..., developed and deposited at Addgene by Arlene Sharpe’s lab, enables in vivo screens in naïve T cells....Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and is interested..., Hacohen, N., Doench, J. G., LaFleur, M. W., & Sharpe, A. H. (2024). Framework for in vivo T cell screens...

  8. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ... the human gene RPE65, patients with clinical diagnosis of LCA and mutations in RPE65 (n=3), showed significant...This disease is caused by mutations in the gene RPE65. It is characterized by childhood onset of blindness...

  9. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...science.adi1910 Chylinski, K., Makarova, K. S., Charpentier, E., & Koonin, E. V. (2014). Classification and...Saunders, S. J., Barrangou, R., Brouns, S. J. J., Charpentier, E., Haft, D. H., Horvath, P., Moineau, S., Mojica... S. A., Alkhnbashi, O. S., Brouns, S. J. J., Charpentier, E., Cheng, D., Haft, D. H., Horvath, P., Moineau...

  11. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ...light using a different objective lens oriented perpendicular to the plane of illumination (i.e. light sheet...Klymkowsky. (1989) Development, 105.61-74. Pubmed. J. Sharpe, U. Ahlgren, P. Perry, Hill B, Ross A, J. Hecksher-Sorensen...

  12. CRISPR 101: Making a Knock-In Cell Line

    Type
    Blog Post
    Published
    Dec. 22, 2022, 2:15 p.m.
    ...potentially shuttling repair down a different pathway (Charpentier et al., Nat Com).     Fig. 3: HDR events... 8(2024), (2017). 10.1038/s41467-017-01836-2 Charpentier, M., Khedher, A. H. Y., Menerot, S., Brion, A...

  13. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...transcriptional activation domain VP16 taken from the herpes simplex virus. tTA binds to promoters containing...Functional dissection of VP16, the trans-activator of herpes simplex virus immediate early gene expression. ...

  14. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...Using acrylamide instead of agarose gives much sharper bands. I compared two MOIs of gRNA transduction... fragments, run PAGE instead of agarose to get sharper bands. Conclusion Overall, making a human cell...

  15. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR Screens...

  19. The Strength of Story Telling

    Type
    Blog Post
    Published
    Dec. 19, 2017, 2 p.m.
    ...learning. Storytelling is a crucial part of interpersonal communication, which makes honing your ability...

  20. Antibodies 101: Epitope Tags

    Type
    Blog Post
    Published
    May 11, 2023, 1:15 p.m.
    ...tagging?   References and Resources References Terpe, K., Overview of tag protein fusions: from molecular...
Showing: 1 - 20 of 51 results