We narrowed to 261 results for: nif

1. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...plasma membrane. Note that the use of the HA tag significantly improves the transport of hM4Di to the plasma...

2. Human Germline Editing Using CRISPR

   Type

   Blog Post

   Published

   Aug. 10, 2017, 2:19 p.m.

   ...biologists and non-biologists alike. Given the public significance of this research, we have the obligation to ...

3. Addgene's Tips for Plasmid Quality Control

   Type

   Blog Post

   Published

   Jan. 14, 2016, 3:30 p.m.

   ...always be derived from a single colony to ensure uniformity of the culture. Sequencing directly from the ...

4. Career Coaching for Scientists: Why and Where Do I Find One?

   Type

   Blog Post

   Published

   Jan. 29, 2015, 1:25 p.m.

   ... for PhDs and postdocs posted by Toronto-based Jennifer Polk of From PhD to Life. She runs great tweet...

5. 5 Steps to Writing Grant Proposals for Grad Students

   Type

   Blog Post

   Published

   Feb. 11, 2014, 12:13 p.m.

   ...strategy The Research Strategy highlights i) the significance and innovation of the project and ii) how you...

6. Popular Retroviral Vectors and Their Uses in Scientific Research

   Type

   Blog Post

   Published

   July 24, 2018, 12:51 p.m.

   ...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

7. Adenoviral Vector Production and Troubleshooting

   Type

   Blog Post

   Published

   June 14, 2018, 11:44 a.m.

   ...years since its discovery, researchers have done significant work to improve the Adv genome’s utility in research...

8. High Complexity Golden Gate Assembly with NEB’s DAD

   Type

   Blog Post

   Published

   Aug. 1, 2023, 1:15 p.m.

   ...of a lac operon dropped to 49% fidelity, with significant lot-to-lot variation. Additionally, this assembly...

9. CRISPR 101: Anti-CRISPR Proteins Switch Off CRISPR-Cas Systems

   Type

   Blog Post

   Published

   July 23, 2020, 1:20 p.m.

   ... May 23, 2017 and last updated Jul 23, 2020 by Jennifer Tsang. CRISPR-Cas technology is constantly evolving...

10. Plasmids 101: In-house NGS Plasmid Quality Control Now at Addgene

    Type

    Blog Post

    Published

    April 6, 2021, 12:30 p.m.

    ...Addgene, each part of this new process involves a significant amount of QC. First, we perform Picogreen quantification...

11. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...doi.org/10.1126/sciadv.abb7438 The CRISPR corner By Jennifer Tsang Here are a few highlights from recent CRISPR...

12. Viral Vectors 101: Calcium Sensors

    Type

    Blog Post

    Published

    Aug. 10, 2023, 1 p.m.

    ...delivered using viral vectors! One of the most significant advantages of viral delivery is the potential...

13. Experimenting in an Open Source lab: from CRISPR to Cats

    Type

    Blog Post

    Published

    July 23, 2015, 2:30 p.m.

    ...interested parties can do research. This interest manifested itself in the creation of Hackerspaces and Makerspaces...

14. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...diseases, as you can target your library to a significant degree using different types of AAV. Expression...

15. Magnetic Control of Proteins: More than a Dream

    Type

    Blog Post

    Published

    Sept. 24, 2024, 1:15 p.m.

    ...Maria’s screens of AsLOV2 variants showed some significant gains! After a few more rounds of mutagenesis...

16. Twenty Years of Addgene Sharing: CRISPR

    Type

    Blog Post

    Published

    April 18, 2024, 1:15 p.m.

    ...other countries, from Japan to Australia, are significant depositors and requestors. CRISPR tools are developed...

17. Tips to Make the Most of a Scientific Conference

    Type

    Blog Post

    Published

    May 23, 2019, 12:26 p.m.

    ... Jennifer Tsang...

18. Mobile-CRISPRi: Bringing CRISPRi to Diverse Bacteria

    Type

    Blog Post

    Published

    April 4, 2019, 12:53 p.m.

    ...Mobile-CRISPRi plasmids at Addgene! References Auchtung, Jennifer M., et al. "Biology of ICEBs1, an integrative ...

19. Of Myc and Men

    Type

    Blog Post

    Published

    Jan. 19, 2023, 2:15 p.m.

    ... the N-terminal half of the antigen differs significantly between human and the other species, suggesting...

20. Chromoproteins: Colorful Proteins For Molecular Biology Experiments

    Type

    Blog Post

    Published

    Feb. 4, 2020, 2:15 p.m.

    ... Jennifer Tsang...