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...understand what metrics would be useful and how to organize the metrics in a digestible manner.  At first,...passed, and I participated in the discussions, I began to note trends in the data, and from that, suggest...

... screens to introduce reporters throughout an organism’s genome. These reporters have historically been...The best trap depends on your experiment and the organism you are working in. The spacing of genes and whether...

...is reflected in the biology, too. CRISPR is an elegant bacterial immune system and an efficient gene editing...distinguishing the foreign sequences and keeping the array organized. Target strand: The strand of foreign DNA that...

...skills”. Leadership is prized at all levels of an organization and is also one of the most common criteria ...work alone much of the time, depending on the organization they lead. Effective leaders rarely give orders...

...valued This often stems from being included in organizational decision-making. It is imperative that employees...understand the part they play in the goals of the organization and feel the importance of their contributions...

...your expression system or environment. The host organism will determine the type of vector that you will...your plasmid has been incorporated into the host organism, usually achieved with the proper selection marker...

...where they are. 8)   Look into training from an organization such as COMPASS. Although COMPASS has a special... Kendall Morgan...

...and is relatively easy to grow, it is an ideal organism for teachers looking to expand their science curriculum...professional education associations, and local organizations such as the Center for Science and Industry ...

... 5’ of the gene they control. Promoters can be organism or tissue type-specific, which can help you restrict....2019.00093 Wilmott P, Lisowski L, Alexander IE, Logan GJ (2019) A User’s Guide to the Inverted Terminal...

...precise titrations of AAV or heat-shocking the organism. The SPARC system uses competitive recombination...system that can be used in many cell types and organisms including Drosophila, fish, and mice, by replacing...

...abundant biological dark matter and so the lab organized a sampling trip to Yellowstone National Park (...then, many researchers have searched for novel organisms and enzymes from these extreme environments. Gearing...

...possible Tell them about the Golden Stopwatch! Any organization that deposits with and/or requests materials...to all of the winners so far! Don’t see your organization below? Don’t worry--we check periodically for...

... etc.) and are ready to use on another cell or organism. This pipeline is utilized to transduce cell lines...resources on the Addgene blog Five Popular Model Organisms 10 Tips for Mammalian Cell Culture Adenoviral...

... about the role these factors played within an organism.  So how did plasmids get their name? In 1952...terminology held until the 1960s when scientists began to study other extrachromosomal particles, particularly...

...Girls) Communication to diverse audiences Organization Mentorship Leadership Science writing...Communication Business models Conference or club organizer Management Leadership Communication ...

...that requires travel can show that you are self organised, reliable, and can work independently. Practical...involves traveling, showing that you are a highly organised and experienced traveler by citing specific examples...

... number of CRISPR plasmids for that particular organism available at Addgene. Percentages represent the...used to deliver CRISPR components to cells or organisms under study. In the past we’ve left the arduous...

...that selection of a fluorescent probe (be it an organic dye or FP) is one of the most important steps in...tools. Years ago, Kurt Thorn (UCSF at the time) began an effort to collate some of the current “best” ...

...If appropriate, invite them to speak for your organization, mentoring group, or department. Even if it ...gestures are memorable! Attend events for one organization or go to the same conferences/meetings over ...

...investigate the susceptibility of cells from different organisms to SARS-CoV-2 (Chu et al., 2020). An additional...mechanisms to maintain and grow these critical organizations should be a fundamental part of the scientific...