We narrowed to 265 results for: nif

1. The PAM Requirement and Expanding CRISPR Beyond SpCas9

   Type

   Blog Post

   Published

   Aug. 20, 2020, 2:30 p.m.

   ...CRISPR guide or leave a comment below. Note: Jennifer Tsang and Mary Gearing contributed to the update...

2. Harnessing TnpB for Genome Editing: A Compact and Versatile Tool for Your Research

   Type

   Blog Post

   Published

   July 29, 2025, 1:15 p.m.

   ... Panda, D., Baig, M. J., & Molla, K. (2025). A unified protocol for genome editing in monocot and dicot...

3. QC Sequencing Technologies at Addgene

   Type

   Blog Post

   Published

   July 15, 2025, 1:15 p.m.

   ...This post was written by Alyssa Shepard, with significant contributions from Alyssa Cecchetelli, Cary Valley...

4. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

   Type

   Blog Post

   Published

   July 8, 2025, 1:15 p.m.

   ...This post was written by Alfonso Timoneda, with significant contributions from Robert Hurt and Mohamed Soufi...

5. Hot Plasmids - November 2023

   Type

   Blog Post

   Published

   Nov. 14, 2023, 3:35 a.m.

   ...efficient for short, unstructured templates and significantly reduced undesired edits (indels, etc.), while...

6. Mesothelioma - Causes, Symptoms, and Treatment

   Type

   Blog Post

   Published

   Dec. 13, 2016, 3:30 p.m.

   ...earlier, thus improving the patient’s prognosis significantly, but also to monitor those who may be at greater...

7. Supporting Reproducibility with a Connected ELN

   Type

   Blog Post

   Published

   Jan. 17, 2019, 1:38 p.m.

   ...with a particular experiment or project thus significantly enhances the ability of others to subsequently...

8. Healthcare Consulting: A Door to the Business of Life Sciences

   Type

   Blog Post

   Published

   Oct. 13, 2016, 2:30 p.m.

   ...is the fact that they occur in the context of significant clinical uncertainties and a rapidly evolving...

9. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...create specific edits in DNA, there has also been significant work on constructing Cas9 transcriptional activators...

10. Bacterial Toxin-antitoxin Systems as Molecular Biology Tools

    Type

    Blog Post

    Published

    Nov. 1, 2018, 12:35 p.m.

    ... Jennifer Tsang...

11. CRISPR 101: Targeting RNA with Cas13a (C2c2)

    Type

    Blog Post

    Published

    Sept. 21, 2017, 2:07 p.m.

    ...displays similar knockdown efficiency but no significant off-target effects, a distinct contrast to the...

12. Plasmids 101: Control Plasmids

    Type

    Blog Post

    Published

    April 9, 2015, 3:29 p.m.

    ...and repeats—what is the difference and is it significant? Vaux DL, et al. EMBO Rep. Apr; 13(4): 291–296...

13. Tips for Using FRET in Your Experiments

    Type

    Blog Post

    Published

    Nov. 5, 2014, 3:41 p.m.

    ...highest absorbing acceptor and fluorophores with significant overlap in their spectra. The pair CFP-YFP was...

14. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type

    Blog Post

    Published

    Aug. 5, 2014, 6:51 p.m.

    ... storing data in a sharable form is indeed a significant effort. Our standards of data recording are highly...

15. CRISPR 101: Validating Your Genome Edit

    Type

    Blog Post

    Published

    Nov. 3, 2022, 12:15 p.m.

    ...clones, it’s wise to quickly assess whether a significant number of cells have been edited. For many experiments...

16. Transferable Skills Guide: Conflict Resolution

    Type

    Blog Post

    Published

    Jan. 4, 2022, 2:15 p.m.

    ...owning. Conflict in this latter scenario usually manifests as the assumption “I thought that was X’s job”...

17. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type

    Blog Post

    Published

    Sept. 1, 2020, 1:15 p.m.

    ..., was 81% of that from a single vector, but significantly greater than the expression from an overlapping...

18. The Advances Behind The World's First Personalized CRISPR Treatment

    Type

    Blog Post

    Published

    Sept. 16, 2025, 1:15 p.m.

    ...double-stranded breaks in the DNA backbone, making them significantly less prone to indel mutations that plagued CRISPR...

19. Antibodies 101: Normalization and Loading Controls for Western Blots

    Type

    Blog Post

    Published

    April 8, 2025, 1:15 p.m.

    ...On this gel, the protein of interest band is significantly lighter in “condition 1” than “condition 2.”...

20. An Integrin Antibody Toolkit from IPI

    Type

    Blog Post

    Published

    March 28, 2024, 1:15 p.m.

    ...kidney development. Despite their ubiquity and significance, these receptors have proved challenging to ...