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We narrowed to 533 results for: arl

Showing: 261 - 280 of 533 results
  1. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...waivers. One of the blog posts that helped me quite early on was Christine Liu’s “How to Become a Scientist...on the Addgene blog Check out our resources for early career researchers Get advice for applying for jobs...
  2. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...requires a nearby PAM, although these are still early days for this technology, and the user may need ... P-Y, Daley G, Church G (2013) Optimization of scarless human stem cell genome editing. Nucleic Acids ...
  3. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...to recognize DNA sequences is not ideal. For the early generation gene editors, new amino acid recognition...engineering of novel base editor proteins. She is particularly interested in combining the realms of art and...
  4. Plasmids 101: Modular Cloning Applications and Kits

    Type
    Blog Post
    Published
    May 14, 2024, 1:15 p.m.
    ...bacteria or cell-free systems. The approach is particularly useful for producing drug precursors, biofuels...works in both mammalian systems and Drosophila. Similarly, the Multi-Kingdom (MK) Golden Gate system was...
  5. Antibodies 101: Flow Cytometry

    Type
    Blog Post
    Published
    July 20, 2021, 1:15 p.m.
    ... different colors. This lends flow cytometry particularly well to complex experiments looking at a variety...from the use of secondary antibodies. It’s also particularly useful for intracellular staining where binding...
  6. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Initial photoactivatable CRISPR systems published in early 2015 focused on using light to control transcription...Moritoshi Sato’s lab at the University of Tokyo and Charles Gersbach’s lab at Duke University developed similar...
  7. CRISPR 101: Cytosine and Adenine Base Editors

    Type
    Blog Post
    Published
    Feb. 13, 2025, 2:15 p.m.
    ...Early CRISPR applications were often limited by the low editing efficiency of homology-directed repair...nickase (Nishida et al., 2016). Target-AID acts similarly but not identically to BE3, modifying a 3-5 base...
  8. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...enough to get good separation in your ladder, particularly in the sizes around your protein of interest... leftover sample) to fill the relevant wells. Similarly, if you load a gel and then forget to start it...
  9. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...provides improvements to classical cloning methods, particularly for complex transgenesis constructs. Kirchmaier...terminator). Next, up to six Level 1 modules can be similarly assembled into a Level 2 vector, thus forming ...
  10. Antibodies 101 Series on TikTok

    Type
    Blog Post
    Published
    March 19, 2024, 1:15 p.m.
    ... Addgene We'll be posting these animations regularly from now until May, so follow us to get updates...
  11. The Twelve Days of Antibodies

    Type
    Blog Post
    Published
    Jan. 6, 2025, 5:17 p.m.
    ...Antibody, Addgene gave to me…  A recombinant Anti-Arl13b!    🎶On the fifth day of Antibody, Addgene gave...
  12. CRISPR 101 eBook...en español

    Type
    Blog Post
    Published
    Jan. 18, 2024, 2:15 p.m.
    ... científica profesional, L. Denise Roche, para editarla. Estamos orgullosos de anunciar que CRISPR 101...
  13. Congrats to the 2019 Deck The Lab Winners

    Type
    Blog Post
    Published
    Jan. 17, 2020, 3 p.m.
    ...tree topper, tube “lights,” and autoclave tape garland. Thanks @ribosholmes!   Frogs and tacos The Miller...
  14. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ...Schaefer, NCI, NIH | License   I’m updating it regularly as people send me suggestions. The most useful...
  15. Twelve Amazing Guest Blog Contributors!

    Type
    Blog Post
    Published
    Nov. 26, 2024, 2:15 p.m.
    ...shared information about recent CRISPR deposits from Arlene Sharpe’s lab. X-Chime and Performing In Vivo CRISPR...
Showing: 261 - 280 of 533 results