We narrowed to 51 results for: codon optimized

Curious about how you can expand the number and type of amino acids available in your experiment? Genetic code expansion can help!

June 15, 2023, 1:15 p.m.

Discover the tools you need to perform plant genome engineering with CRISPR.

Oct. 11, 2016, 2:30 p.m.

Scientists are now using Addgene's plasmid repository as a source of sequence data sets. Researchers from MIT built a classifier that predicts whether a gene sequence is natural or synthetic and found that more and more genes are being sourced from farther away in the tree of life.

Nov. 8, 2018, 3:47 p.m.

An introduction to transposons including Sleeping Beauty, piggyBac, and Tol2 and how they can be used in transposon mutagenesis screens, gene transfer, and creating transgenic animals.

July 6, 2021, 1:28 p.m.

One-pot saturation mutagenesis can quickly generate libraries of mutant plasmids for phenotypic screening. Learn more from SciComm intern Beth Kenkel.

Feb. 22, 2017, 3:30 p.m.

Recent highlights from Addgene's collection include a system to examine biosynthetic pathways, and a new tool for enriching ABE-targeted cells.

Sept. 21, 2021, 1:15 p.m.

Use these CRISPR base editing reporters to track base editing events in real-time and enrich for base edited cells.

July 7, 2020, 1:15 p.m.

Learn about hot new tools in our collection!

Feb. 17, 2026, 2:15 p.m.

Check out all of the plasmids featured in our hot plasmids articles from 2016.

Dec. 22, 2016, 3:03 p.m.

Learn about the csRNP epitope tagging method - a new and efficient way to tag endogenous genes using CRISPR.

June 28, 2018, 3:01 p.m.

CRISPR genome editing often proceeds through NHEJ or HDR but microhomology-mediated end-joining (MMEJ) has many benefits - read about them here.

Feb. 23, 2016, 3:30 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

Learn more about E. coli strains used for protein overexpression.

Feb. 10, 2015, 3:06 p.m.

Read on for a sampling of some of the exciting new plasmid technologies that have come through our doors in 2015.

Dec. 23, 2015, 3:30 p.m.

Guest blogger Pooran Dewari presents results of an NgAgo survey he gave to some of the first NgAgo users. Find out how NgAgo compares to Cas9 so far.

Aug. 4, 2016, 2:30 p.m.

A new open source tool for CRISPR experiments, CrispyCrunch, has been developed by the Chan Zuckerberg Initiative to design batch gRNAs, templates, and sequencing primers for CRISPR + HDR experiments.

Feb. 7, 2019, 2:16 p.m.

Learn about antibody engineering, including what it is and why we like it. Deep dive into antibody directed evolution techniques, and learn about Addgene's partnership with the Institute for Protein Innovation (IPI).

Aug. 5, 2025, 1:15 p.m.

Learn about the sources of spontaneous recombination and optimizing Cre-recombinase dependent AAV vector expression in vivo.

Nov. 2, 2021, 1:15 p.m.

Practical advice for using CRISPRs: designing your gRNA and introducing it into cells.

Sept. 24, 2020, 1:15 p.m.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

June 18, 2020, 1:15 p.m.