We narrowed to 23 results for: gibson assembly

1. Plasmids 101: Colony PCR

   Type

   Blog Post

   Published

   May 12, 2016, 2:30 p.m.

   ...Restriction Cloning Try SLIC cloning Learn about Gibson Assembly Additional Resources on Addgene.org Read ...

2. CRISPR Methods for Bacteria: Genome Engineering, CRISPRa, CRISPRi, Base Editing, and More

   Type

   Blog Post

   Published

   Sept. 28, 2020, noon

   ...extra elements, like a repair template, using Gibson Assembly or restriction enzyme cloning. Streptomyces...are easily inserted using BbsI and Golden Gate Assembly. Either plasmid can be also linearized with XbaI...Microbiome Bacteria with a Toolkit for Modular Assembly of Broad-Host-Range Plasmids. ACS Synth Biol 7...

3. Recombinase-based State Machines Enable Order-dependent Logic in vivo

   Type

   Blog Post

   Published

   July 28, 2016, 2:30 p.m.

   ... recommend using overlap extension PCR and Gibson assembly to piece together the registers. All parts ...