Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
Type
Blog Post
Published
July 26, 2018, 12:46 p.m.
...fused to a bipartite nuclear localization signal (bNLS) and a nuclear export signal (NES), both which are...localizes to the nucleus, but following phosphorylation bNLS activity is suppressed and NES activity is enhanced...positions of the phosphorylatable residues and the bNLS and NES sequences, which have previously been characterized...Kudo et al. for more details on the design of the bNLS and NES sequences.
Disadvantages of KTRs
KTRs also...