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We narrowed to 789 results for: OMP;

Showing: 501 - 520 of 789 results

  1. CRISPR 101: Homology Directed Repair

    Type
    Blog Post
    Published
    Jan. 26, 2023, 2:15 p.m.
    ...recombination intermediates can then resolve to complete the DNA repair process Homology directed repair...is elongated and ligated to fill the gap, thus completing SDSA.   Break-induced repair (BIR) pathway BIR... uses reverse transcriptase to generate the complementary ssDNA. Easi-CRISPR was shown to increasing editing...

  2. 10 Steps to a Perfect Science Talk

    Type
    Blog Post
    Published
    Aug. 23, 2016, 2:30 p.m.
    ...get people to remember you). What better way to accomplish all of these things naturally than to give a ...simplicity of their slides and how the slides complement the spoken message. Learn from their level of... topics that are unfamiliar to you because the company you are visiting works on them. Of course, if some...

  3. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...regularly. Dystrophin and genome editing Since complex oligonucleotide treatment comes with many challenges...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period... with vastly reduced inflammation and necrosis compared to unedited animals. In grip strength, specific...

  4. Evolution of Brainbow: Using Cre-lox for Multicolor Labeling of Neurons

    Type
    Blog Post
    Published
    April 24, 2015, 2:39 p.m.
    ...system, Sanes and Lichtman also developed a complementary Flpbow construct that is functionally similar...technique will provide important insights into the complicated physical organization of the brain and other ...Intestinal crypt homeostasis results from neutral competition between symmetrically dividing Lgr5 stem cells...

  5. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut... accessibility due to chromatin status). No computational prediction is ever perfect, but this can decrease...to conduct genetic screens, however, a library composed of the latter would likely be more effective, ...

  6. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...stimulation. GPCR signaling GPCR signaling is a complicated process involving many downstream signaling mechanisms...applying optogenetic tools derived from direct comparative analysis of microbial opsins. Nature Methods,...

  7. Building Global Connections with the International Mentorship Program USA-EUROPE

    Type
    Blog Post
    Published
    Nov. 6, 2015, 3:30 p.m.
    ... a post-program self-assessment, participants completed follow-up self assessments and program evaluations...knowledge and skills had improved significantly when compared to their pre-program self-assesment. Achievements...other countries. In 2016, we expect the program to comprise over 2000 participants (mentors and mentees). ...

  8. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...validation is so important. Antibodies are a critical component of the researcher toolkit due to their ability...will bind to - particularly when you start using complex samples such as whole cell lysate or tissue sections...BioRender.com.     Orthogonal Strategies This approach compares target expression of antibody-independent approaches...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...transfer expert who understands the somewhat complicated Material Transfer Agreement process that kits...function. Project team A Project Team is assembled to complete a series of tasks with precisely defined goals...organization on the planet, in which case whoa! Mission accomplished! One example of a mission-based team in academia...

  10. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...In 1976, the world’s first genetic engineering company, Genentech, was founded.  This commercialization...instead of recognizing nucleotide triplets, the components recognize individual nucleotides. This imparts...intracellular pangenesis to the rediscovery of Mendel. Comptes Rendus de l’Académie des Sciences - Series III ...

  11. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...attached fluorophore. However, since tRNA suppression competes with natural chain termination at stop codons,...contrast can be improved using disulfide-containing compounds such as British anti-Lewisite (which was originally...Vilensky & Redman, 2003]). Most importantly, these compounds cross cell membranes, thus enabling FRET measurements...

  12. CRISPR/Cas9 FAQs Answered!

    Type
    Blog Post
    Published
    March 13, 2014, 4:08 p.m.
    ...system, developed by the Zhang lab, which has comparable efficiency to the optimized chimeric design but...Where are double strand breaks (DSBs) induced, compared to where the target site sequence (protospacer...how closely homologous the off-target sites are compared to the on-target site, the specific site sequence...

  13. Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More

    Type
    Blog Post
    Published
    Sept. 8, 2017, 7:52 p.m.
    ... this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly generating zebrafish transgenic.... Listen to Our Segement on the Tol 2 Gateway-Compatible Toolbox  Hot Plasmid 2: AAV Vectors for tageting...

  14. DIY DNA Ladders from Penn State University

    Type
    Blog Post
    Published
    July 14, 2017, 2:30 p.m.
    ...Get Tips on Verifying Your Plasmid Table 1: Comparing Typical Comericial Ladders with the PSU Ladders...1000 Lanes Cost $200-$500  <$10 $20-30 Cost Comparison: While convenient, commercial ladders cost between...

  15. Antibody Data Repositories and Search Engines

    Type
    Blog Post
    Published
    Oct. 31, 2024, 1:15 p.m.
    ...experiment. Search engines let you easily find and compare available antibodies from many vendors, while data...Search engine May include validation data  Biocompare Any Any Search engine     Are we missing...

  17. Streaking for Single Colonies: The Streak Plate Challenge

    Type
    Blog Post
    Published
    March 2, 2021, 2:15 p.m.
    ... 1. Lab B needs a redo :-( @SandTBioSci #CompetitiveStreaking @Addgene pic.twitter.com/mk44J4Gcj9 — Dave...species for the challenge, but one species always outcompeted the other species.  When he found the chromoprotein...

  18. CRISPR Cheat Sheet

    Type
    Blog Post
    Published
    Jan. 27, 2021, 2 p.m.
    ...genome usually for a research or applied goal. Components of CRISPR: gRNAs: Sequences of RNA that direct...single base changes in the DNA sequence without completely cutting the DNA. RNA Editors: CRISPR nucleases...

  19. 2018 #DeckTheLab Contest Winners

    Type
    Blog Post
    Published
    Jan. 15, 2019, 2:19 p.m.
    ...decorated office! Below, they turned one of the lab computers into a fireplace scene.  Got our @EmoryChem ...all who participated in the 2018 #DeckTheLab competition. The four winners will receive a holiday care...
Showing: 501 - 520 of 789 results