We narrowed to 804 results for: OMP;

...custom primers or "primer-walking" reactions are completely unnecessary for sequencing multi-kb DNA inserts...seqWell, Inc. He is a sequencing technologist and computational biologist with a passion for solving critical...

... production of viruses used to deliver CRISPR components to cells or organisms under study. In the past...lentivirus production frustrating - a researcher can complete an entire lentiviral production protocol only ...

...would recognize serotonin. Using a combination of computer modeling, directed evolution, site-saturation ...and colonization opportunities for bacteria. To compare the artificial leaf to A. thaliana leaf, Bernach...

...and sgRNA-Sirius-8XMS2 scaffolds performed, they compared them head-to-head against CRISPRainbow. Both tools...visualization tools can be used in live cells. Compared to its predecessor CRISPRainbow, CRISPR-Sirius...

...techniques are continually being refined; it is completely plausible that scientists will eventually have...standardized process of good research practice: computer simulations, in vitro research, animal models,...

...playing a card game called “Magic”. There were complicated rules that governed how this game was played....should be “brains-on”, but as much as I try being compelling, interactive, and application-driven to boost...

...Musicophilia is accessible and fascinating, all without compromising on the relevant science."     - Randall Carter...Additional resources on the Addgene blog Learn about company culture at Addgene Read about other lunch activities...

...link can be your new card. Follow your contact’s company on LinkedIn (or via industry newsletters such as...this because I want it to be clear that it is completely impossible for me or anyone to stay in touch ...

...field. Of course they are competing with each other, but it's a healthy competition. What an honor for Addgene...

...and learn from them. I started at my previous company by taking on responsibilities such as mentoring...facilitated a team meeting to highlight their complementary strengths. By encouraging alignment on goals...

...shRNAs are short RNA sequences which target complementary mRNA sequence for degradation. Viral introduction... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...

... creates a non-functional 𝛽-galactosidase. Complementing this mutation by expressing this deleted portion...get started, you will first need a primer that complements your plasmid sequence. Start with a backbone-...

...increase in the number of endogenous indel edits when compared to unsorted cells (Wen et al., 2018). Thus SRIRACCHA...expression. As a proof of concept the Denham lab compared editing at three different genomic sites in the...

...SSAP system reports an efficiency of up to 20%, comparable to commonly used CRISPR/Cas9 methods for large...error rates overall ranged from 0.3% to 10%, in comparison to the 62-90% error rate seen with Cas9 editors...

... and make a checkmark next to each step as you complete it. Yes, this can be incredibly tedious, but it...several steps into one. Soon you’ll figure out what prompts you need to consistently and accurately run a protocol...

...portion of different antibody isotypes. Binding to complement system proteins, which can initiate a cascade...antibodies through cells and into different body compartments, such as into mucus, tears, or milk. When using...

...lentiviruses expressing various gRNAs. It’s better to compare the phenotypic effects of expressing various gRNAs...expression between experiments and over time (as compared to separate transductions with a single-vector...

...resulting in a Cas9 with improved specificity. They compared on-site and off-site activity of Sniper-Cas9 to...et al. "Evolved Cas9 variants with broad PAM compatibility and high DNA specificity." Nature 556.7699 (...

...binding of a new inactive heterotrimeric G protein complex and subsequently another round of signaling.    ...gamma subunits of the heterotrimeric G protein complex that can be used to detect G protein activation...

... range from a defined pathway or reaction to a complex event such as cancer or even aging. For both a ...CRISPR, drug, etc.), just with an added baseline component. Alternative screening techniques While CRISPR...