We narrowed to 793 results for: lat
-
TypeBlog PostPublishedJune 10, 2025, 1:15 p.m.... systems; Cascade shown) combines with a donor template and transposon proteins (Tns) to integrate large...outcompete their brethren, and the researchers isolated these more efficient CASTs (Witte et al., 2025...
-
New Viral Vectors - Fall 2024
TypeBlog PostPublishedOct. 3, 2024, 1:15 p.m....endothelial cells via an injection into the systemic circulation. In Old World monkeys it actually targets neurons...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Year of the Camelid: Antibody Style
TypeBlog PostPublishedMay 9, 2024, 1:15 p.m....cultural importance of these animals to human populations around the world. Here at Addgene, we love camelids...living cells or tissues. sdAbs can also be used to manipulate cellular functions, like in this early example... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....proteins are also involved in the transcriptional regulation of the AAV p40 promoter which drives Cap expression...promoter. Alternative splicing and differing translational start sites generate the three VP proteins. ... -
Sleeping Beauty Awakens for Genome Engineering
TypeBlog PostPublishedJune 30, 2015, 2 p.m....use of viruses is also important for social and regulatory reasons. 1.) The transposon consists of a mirrored...your gene-of-interest, either constitutively or regulated by doxycycline. The Sleeping Beauty transposase... -
Choosing Your Perfect Empty Backbone
TypeBlog PostPublishedAug. 19, 2014, 3:39 p.m....backbones) are frequently used in molecular biology to isolate, multiply, or express the insert they carry in ... system is great for overexpressing YGOI in an isolated, controlled environment. In most cases bacterial... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....even by introducing a person that the audience can relate to. It doesn’t need to be anything fancy; it just... anthropomorphized animals, or even seemingly unrelated stories that they then tuck the science into. ... -
Addgene Receives NIH BRAIN Initiative Grant to Create Open-Access Recombinant Antibody Resource
TypeBlog PostPublishedMay 25, 2021, 2:29 p.m.... focus of the NABOR platform will be neuroscience reagents and data, the platform can be expanded for ... -
What Do I Do Now? Academic v. Non-Academic Career Decisions
TypeBlog PostPublishedMarch 9, 2017, 3:40 p.m....scheduling Diverse opportunities to do science related jobs that require no bench work Better resources... important first step. Developing mentoring relationships, especially with peer mentors, to discuss your... -
Viral Vectors 101: What is a Virus (and a Viral Vector)?
TypeBlog PostPublishedAug. 18, 2023, 4:15 p.m....genomes can be transcribed into mRNA, directly translated into proteins, or, in the case of retroviruses...which is then transcribed into mRNA before being translated into proteins. RNA viruses replicate their genomes... -
Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation
TypeBlog PostPublishedDec. 13, 2018, 1:14 p.m....sorbitol is already a common excipient in AAV formulation buffers, this sugar may be a more attractive ...Charlotte A., Michael Hollinshead, and Amin Hajitou. "Clathrin-mediated endocytosis and subsequent endo-lysosomal... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...viral vector repository with a nice cup of hot chocolate is absolutely delightful. Grab a hot beverage ...forget to use the link below to keep up with the latest in Addgene's viral vector services and resources... -
Science Careers: Unruly Interests Feed Many Paths
TypeBlog PostPublishedSept. 26, 2017, 12:12 p.m.... projects studying developmentally programmed regulation of DNA replication and gene transcription during... useful and productive, forming a different constellation of interests and skills than what fits for applied... -
HA Frankenbody, a New Imaging Tool to Visualize Single Molecules and Nascent Peptides
TypeBlog PostPublishedOct. 10, 2019, 12:41 p.m....single mRNA translation in U2OS cells. HA is small, only 9 amino acids, thus it is translated quickly by... -
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....diseases, offering them the chance to win some of the latest tools and technologies in life sciences research...affected by AHC suffer from recurrent attacks of unilateral paralysis (hemiplegia) that are often painful... -
Don’t Wait To Launch Your Health Science Startup
TypeBlog PostPublishedAug. 25, 2015, 2:30 p.m....companies. The majority of these meetings acted as a platform for significant company advancement. Be relentless... you’re able to answer every possible question related to it. First impressions are everything and you... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....the same myths and misconceptions fifteen years later. What does that look like? One of my more recent...designs could work to slow the spread of illness. I later helped run workshops where I showed people how they... -
New Neuroscience Tool: The iGluSnFR3 Glutamate Sensor
TypeBlog PostPublishedApril 7, 2022, 1:15 p.m....trigger other forms of neuronal signaling and regulation. To create iGluSnFR, the Looger lab wedged GFP...soluble protein CAG: Generic strong promoter. Formulated as AAV-DJ for neuronal expression. CAG-FLEX: ... -
Pushing the Limits of DNA Assembly
TypeBlog PostPublishedSept. 6, 2017, 1:01 p.m....DNA – perfect for transformation or as a PCR template. Some of the applications of the NEBuilder HiFi...plasmids can be directly transformed into E.coli for plating, screening and generation of sgRNA. Generation ... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....when Gal4 is expressed. Gal4 expression can be regulated by any chosen promoter, and Bloomington’s Stock...make your own. In my next post, I’ll explain the relatively straightforward process of generating your own...