968 results

The tools of optogenetics can be combined with CRISPR to generate inducible genome edits and gene regulation systems.

Sept. 3, 2020, 12:15 p.m.

Overview of split vector approaches for fitting genes >4.7 kB into AAV including overlapping, transplicing, hybrid, and sequential homologous recombination.

Sept. 1, 2020, 1:15 p.m.

Bimolecular fluorescence complementation is a method that uses split fluorescent proteins tethered to two potential protein interaction partners.

Aug. 27, 2020, 1:15 p.m.

Protospacer Adjacent Motifs (PAM) serve as a binding signal for Cas proteins. Here we highlight the many Cas proteins and their PAM sequence variations.

Aug. 20, 2020, 2:30 p.m.

Learn about new plasmids and viral preps available at Addgene including a yeast-based tool for protein evolution, fluorescent tools, nanobody purification, new CRISPR tools, and calcium and norepinephrine biosensors.

Aug. 18, 2020, 1:15 p.m.

Addgene marketing intern, Brandon Solis, shares his experience working virtually in a COVID-19 world and his Meet an Addgenie Instagram project.

Aug. 13, 2020, 1:21 p.m.

Learn about the basic elements that make up the AAV transfer plasmid, including the ITRs, transgene, promoter, and other cis-regulatory elements.

Aug. 11, 2020, 1:15 p.m.

Repositories provide fast access to wet lab research materials during COVID-19 such as cell lines, plasmids, and mouse strains.

Aug. 4, 2020, 1:15 p.m.

REPAIR and RESCUE are two CRISPR tools for RNA editing based on Cas13b.

July 31, 2020, 12:30 p.m.

Marc Zimmer's book, The State of Science, examines broad issues in science including racism and sexism, peer-review, funding, CRISPR, and the politicization of science.

July 30, 2020, 1:15 p.m.