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We narrowed to 298 results for: Nov

Showing: 61 - 80 of 298 results

  1. Addgene-Seeding Labs plasmid grant round 3: microbial drug resistance and biofilms, antidiabetic plant components, visualizing brain injury - and a glowing robot fish

    Type
    Blog Post
    Published
    Nov. 27, 2018, 2 p.m.
    ...activity after ischemic brain injury Dr. Gohar Tsakanova, Institute of Molecular Biology of the NationalAcademy...Sciences of the Republic of Armenia, Armenia Gohar Tsakanova is a Senior Researcher who investigates the molecular...become blocked, resulting in reduced blood flow. Novel treatment options, as well as non-pharmacological...two-photon laser scanning microscope will allow Tsakanova to image cortical neurons and study changes in...

  2. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...CRISPR–Cas9 activity switching via a novel optogenetic variant of AcrIIA4 (CASANOVA). As shown in the figure below...release of Cas9. A noteworthy application of the CASANOVA system was its use in light-mediated CRISPR labeling...minutes and red foci formed 20-40 min later. The CASANOVA system is versatile in that it can be adapted ...Light-Dependent Anti-CRISPR Bubeck et al., 2018 CASANOVA LOV2 from A. Sativa AcrIIA4 anti-CRISPR protein...

  3. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...Commun. 2017. PubMed PMID: 28646204. soCoChR: A novel opsin that enables single-cell optogenetics Article...system. Taken together, Shemesh et. al. describe a novel system for the precise mapping of neuronal networks...developed two new FLIM-FRET acceptors, ShadowG, a novel dark green fluorescent protein (GFP) with an absorption...absorption peak of 486 nm and ShadowY, a novel dark yellow fluorescent protein (YFP) with an excitation...

  4. Adeno Associated Virus (AAV) for Cell and Gene Therapy

    Type
    Blog Post
    Published
    Nov. 7, 2017, 1:59 p.m.
    ...have been investigated including lentiviruses, adenovirus, and inorganic delivery tools such as nanoparticles... AAV was first discovered as a contaminant of adenovirus preparations. It is an icosahedral, non-enveloped... requires co-infection of helper viruses like adenovirus for productive infection. AAV vectors are engineered... have slight variations in their viral capsids. Novel forms of AAV capsids are discovered frequently and...

  5. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... Cox DBT, Shmakov S, Makarova KS, Semenova E, Minakhin L, Severinov K, Regev A, Lander ES, Koonin EV, ...Wolf YI, Gootenberg JS, Semenova E, Minakhin L, Joung J, Konermann S, Severinov K, Zhang F, Koonin EV (...

  6. Designing Your Chalk Talk for the Academic Job Interview

    Type
    Blog Post
    Published
    March 12, 2019, 1:37 p.m.
    ...research? Do you have innovative tools? A novel dataset? Unique expertise? A novel hypothesis? Part of ...problem and then explain why it’s significant and innovative. For example, "while it is possible to grow liver...

  7. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...Development - Steven C. Almo, Institute for Protein Innovation  1:18:45 - 2:01:18 (end) - Panel Alex Tucker... talk about work at the Institute for Protein Innovation (IPI) (49:45 - 1:18:45), there are also initiatives...conflict with our original hypotheses. Looking for novelty in our stories, we are additionally averse to producing...productive criticisms that focus less on perceived novelty and impact. Journals could also begin requiring...

  8. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ... encourage people to go to Addgene to get new innovative tools. Afterall, isn’t science better when we...fluorescent proteins has allowed researchers to make innovative tools not possible with other luciferases. In...luciferase reporter from a deep sea shrimp utilizing a novel imidazopyrazinone substrate. ACS Chem Biol 7, 1848...PMC5171807. 6. Machleidt, T., et al. (2015) NanoBRET-A novel BRET platform for the analysis of protein-protein...

  9. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...Recommended Reading Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. (2023). Various AAV...s41434-019-0075-6 Issa, S. S., Shaimardanova, A. A., Solovyeva, V. V., & Rizvanov, A. A. (2023). Various AAV...

  10. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...-chimeras) is that they undergo normal protein turnover. FP-chimeras are continuously synthesized and ...virtually impossible to determine specific protein turnover or temporal expression using standard FP-chimeric...stability, chromophore maturation rate at 37 °C, turnover rate, and the level of background fluorescence...scientists can track single protein localization, turnover, and trafficking, as well as organelle trafficking...

  11. Embracing Serendipity: A Crucial Element in the PhD Journey

    Type
    Blog Post
    Published
    April 25, 2024, 1:15 p.m.
    ... a diverse range of perspectives and sparking innovative thinking that may benefit your current project...mindset that can lead to unexpected discoveries and innovative solutions. Allowing for serendipity in your lab...confines of a predefined path, opening doors to novel ideas and approaches. Moreover, the ability to embrace...

  12. History of CRISPR Cas - A tale of survival and evolution

    Type
    Blog Post
    Published
    Nov. 25, 2013, 10:12 p.m.
    ...destroy the invader. Wikipedia, accessed 25 November 2013. Author: James Atmos (2). Many CRISPR/...programmable platform well-suited for various innovative biological and translative technologies. Addgene...(12): 5429–33. Photo: Wikipedia, accessed 25 November 2013. Author: James Atmos. http://en.wikipedia.org...

  13. Why Add Sucrose? Improved Yields for Adeno-associated Virus Preparation

    Type
    Blog Post
    Published
    Dec. 13, 2018, 1:14 p.m.
    ...different viral production strategies or developing novel methods for data analysis, our team’s goal is to...Palsson, 1991; Oh, 1993) and increased yields in adenoviral (Coroadinha, 2006) and retroviral production ...phase, but not the production phase, improves adenovirus production." Journal of biotechnology 157.1 (...

  14. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...Netherlands. Transformation of HEK cells with adenovirus 5 DNA was postdoc Frank Graham’s 293rd experiment...transformation of human neuronal cells by human adenoviruses and the origin of HEK 293 cells. FASEB J. (2002...Organisms 10 Tips for Mammalian Cell Culture Adenoviral Vector Production and Troubleshooting Additional...

  15. Celebrate National Book Month with Addgene

    Type
    Blog Post
    Published
    Oct. 3, 2019, 12:47 p.m.
    ...is the first 3 books of a larger epic of fantasy novels. Set in a world where 1000 years ago the hero lost...     - Jason Snair, Product Designer Sarum: The Novel of England by Edward Rutherfurd "A 10,000 year history...undergraduate degree in English, I had lapsed. Baker’s novel reignited my appreciation of poetry, and I’ve been...

  16. Photosensitizer Induced Cell Ablation with FAP-TAP MG-2I-dL5**

    Type
    Blog Post
    Published
    Sept. 19, 2017, 1:20 p.m.
    .... M., Britanova, O. V., Yanushevich, Y. G., Staroverov, D. B., Chepurnykh, T. V., . . . Lukyanov, K. A...Fluorescent Protein Guide Page Check out MiniSOG2 and SuperNova, two other photosensitizers used for cell ablation...

  17. Addgene Depositors Get More Citations

    Type
    Blog Post
    Published
    April 20, 2017, 2:30 p.m.
    ...She is particularly interested in exploring how innovative tools influence the direction of scientific research...    Neil Thompson is an Assistant Professor of Innovation and Strategy at the MIT Sloan School of Management...performance, and randomized control trials in innovation. Visit his website at: http://www.neil-t.com/...

  18. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...includes four promoters, six fluorophores with nonoverlapping emission spectra (N- and C-terminal tags for...using these tools in virtually any vertebrate. This novel AAV approach allows you to target and functionally...Podcast Segment on This New AAV Tool    mCyRFP1: Novel fluorescent protein that enables imaging of two ...Lin and Ryohei Yasuda have teamed up to develop a novel monomeric cyan-excitable red fluorescent protein...

  19. AAV Q&A with Tim Miles

    Type
    Blog Post
    Published
    May 17, 2022, 1:15 p.m.
    ...user Resources on Addgene.org Find Adenovirus Plasmids Adenovirus Guide Page Subscribe to Viral Vectors...virus in your research - a primer for beginners Adenoviral delivery of CRISPR/Cas9 Tips for a first time...

  20. Targeted Mutagenesis with EvolvR

    Type
    Blog Post
    Published
    Feb. 21, 2019, 1:15 p.m.
    ...Berkeley, provides a new approach for generating novel genetic variants in bacteria. Read on to learn about...of EvolvR The Dueber Lab used EvolvR to discover novel genotypes that result in a spectinomycin resistant...spectinomycin, rpsE. These results not only uncovered novel resistance genotypes, but also provide a better ...
Showing: 61 - 80 of 298 results