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We narrowed to 258 results for: nif

Showing: 61 - 80 of 258 results

  1. Illuminating Epigenetics with A FRET Based Biosensor

    Type
    Blog Post
    Published
    Nov. 19, 2015, 3:30 p.m.
    ...study,epigenetic changes were statistically significantly associated with reduced life-span, but no changes...controlled, the reporter consistently showed significantly different FRET levels between unmethylated and...

  2. Light Sheet Fluorescence Microscopy

    Type
    Blog Post
    Published
    July 16, 2015, 2:30 p.m.
    ... milliseconds to acquire one image, thereby significantly reducing the time it takes to image through ...Similar to light microscopy, there have been significant advances in tissue clearing techniques during...

  3. Uncovering Ancestral Bias in CRISPR Research

    Type
    Blog Post
    Published
    Nov. 29, 2022, 2:15 p.m.
    ...ancestry, Sean and Aaron knew that their study had significant statistical limitations, and expected to find...Cas9 from cutting, both of which can introduce significant bias into the results. But the reference genome...

  4. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...posits the way science is being done has changed significantly over the last decade. He describes the detection...these fields, and seeing names like Feng Zhang, Jennifer Doudna, Karl Deisseroth, and Ed Boyden crop up...

  5. The Effect of COVID-19 on Liu Lab Plasmid Requests From Addgene Data

    Type
    Blog Post
    Published
    July 16, 2020, 1:15 p.m.
    ...months, pausing or terminating employment for a significant fraction of the workforce. Wet lab scientists...exponential decay model, with dips during weekends and significant holidays. Figure 4: Plasmid request trajectories...

  6. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... channel as the Cas13 reporter), they could significantly amplify the detection signal for a single target...but is undergoing validation studies.     Jennifer Tsang contributed to updating this article. References...

  8. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ...expressing it with additional cofactors or using significantly truncated forms that limit its functionality...Both PILATeS (18 kDa) and STReTCh (11 kDa) are significantly smaller than FRET-based mechanosensors (~60 ...

  9. Twenty Years of Sharing: Addgene's Viral Vector Service

    Type
    Blog Post
    Published
    July 30, 2024, 1:15 p.m.
    ... the Addgene repository can save researchers significant time and removes a variable from the lengthy ...trends in our own repository data, giving us significant insight into which vectors are likely to be of...

  10. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ...Ward T.H., Margison G.P. “Mycoplasma infection significantly alters microarray gene expression profiles.”...Ellem K.A., Cleghorn G., West M.J., Wei MQ. “The significance of controlled conditions in lentiviral vector...

  11. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...tdTomato), they produce a temperature sensor that significantly outperforms previous designs. B-gTEMP is excitable...optimal conditions). While B-gTEMP itself is a significant improvement over previous temperature indicators...

  12. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...has spread to almost every part of the world manifesting as a full-fledged pandemic. Containing the spread...emission reader Approach: FnCas9 Editor Linked Uniform Detection Assay (FELUDA) is a technique that uses...

  14. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...are not exclusive to IF, but they can have a significant impact on the outcome of your IF experiment. ...antibodies you choose for your experiment will have a significant impact on the protocol you use and the ultimate...

  15. Replacing Paper: Tips for Choosing an Electronic lab Notebook

    Type
    Blog Post
    Published
    July 10, 2018, 1:08 p.m.
    ...survey respondents indicated that cost was a significant barrier to ELN adoption. This includes financial... step of the research process will save them significant amounts of time and effort and give the lab a...

  16. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...mice, it is sex-independent and does not show significant differences in transduction efficiency between...brain. Both AAV9 and AAV9.452sub.LUNG1 have significantly higher off-target expression in the liver than...This post was written by Rachel Leeson, with significant contributions from Cynthia Arokiaraj. Addgene...

  17. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...results, the signal from your target needs to be significantly higher than the background noise (Waters 2009....14112. PubMed Central PMCID: PMC5773976. Waters, Jennifer C. “Accuracy and Precision in Quantitative Fluorescence...

  18. PiggyBac-ing Through the Genome Editing Field

    Type
    Blog Post
    Published
    May 31, 2016, 3:30 p.m.
    ...of interest. The piggyBac™ system allows for significantly enhanced random integration efficiencies that... If the cell’s chromatin structure undergoes significant rearrangement (such as when a stem cell initiates...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts to do so have...improved version of BioID, called BioID2, is significantly smaller, allows for more selective targeting...metabolically cooperating (SeMeCo) communities. The uniform multiple cloning site in the plasmid series also...

  20. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ... Organizing a thoughtful engaging talk takes significant effort. It requires evaluation of all of the ...University, did his postdoctoral fellowship with Jennifer Lippincott-Schwartz at the National Institutes...
Showing: 61 - 80 of 258 results