961 results

Learn about the different types of stem cells in this guide. In particular, we discuss the pros and cons of using embryonic stem cells and adult stem cells and using induced pluripotent stem cells (iPSCs) as a model for regenerative medicine.

Oct. 30, 2018, 1:04 p.m.

Find tips for transforming arabidopsis with your plasmid construct of interest.

Oct. 25, 2018, 1:23 p.m.

Get tips for producing, purifying, and tittering AAV, a tick but useful viral vector of choice for many people working to express genes in mammalian cells.

Oct. 23, 2018, 11:49 a.m.

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, gives advice on how to complete a successful postdoc and set yourself on a path to a successful academic career.

Oct. 9, 2018, 12:21 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Get tips on choosing the best lab for your graduate or postdoc work.

Oct. 2, 2018, 12:56 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Addgene has recently reached the 1 million plasmids shared milestone. This post shares some of the social media around this feat and answers the questions: how much space does 1 million vials containing plasmids take?

Sept. 25, 2018, 2:39 p.m.