968 results

In Vivo Assembly (IVA) cloning uses the bacterial recombination pathway to allow any cloning procedure to be performed with a simple two-step, 2 hr protocol prior to transformation. This post covers the basics of the IVA cloning procedure, primer design, and tips & tricks for successful IVA cloning.

Oct. 18, 2018, 12:37 p.m.

Learn a variety of techniques you can use increase the fidelity of multi-part Golden Gate Assembly reactions.

Oct. 11, 2018, 12:30 p.m.

Erik Snapp, Director of Graduate and Postdoctoral Programs at Janelia Research Campus, gives advice on how to complete a successful postdoc and set yourself on a path to a successful academic career.

Oct. 9, 2018, 12:21 p.m.

The labs of John Doench and David Root have recently created CRISPR libraries to knock out, inactivate, or activate genes for genome-wide screens. These optimized libraries are effective for probing gene function while using fewer sgRNAs.

Oct. 4, 2018, 12:44 p.m.

Get tips on choosing the best lab for your graduate or postdoc work.

Oct. 2, 2018, 12:56 p.m.

Learn why codon usage bias exists and techniques you can use to avoid the detrimental affects of codon usage bias on heterologous protein expression - codon optimization and expression of alternative tRNAs.

Sept. 27, 2018, 1:09 p.m.

Addgene has recently reached the 1 million plasmids shared milestone. This post shares some of the social media around this feat and answers the questions: how much space does 1 million vials containing plasmids take?

Sept. 25, 2018, 2:39 p.m.

Learn how the Personal Genetics Education Project (pgEd) engages with various communities on biological science and technology. Get tips on how to work at a similar organization from pgEd's director of new initiatives, Johnny Kung.

Sept. 20, 2018, 12:17 p.m.

Learn all about how to regulate cell growth dynamics with optogenetics, the Riboglow platform for visualizing RNA production and localization, mCarmine, a new and improved red fluorescent protein, and the ACE reporter system for quantifying base-editing and Cas9 cleavage activity.

Sept. 18, 2018, 1:33 p.m.

Genome-wide CRISPR/Cas9 screens are a high-throughput systematic approach for identifying genes involved in a biological process. Recently the Bassik Lab at Stanford developed a new genome-wide CRISPR knockout screen to analyze unanswered questions about CRISPR screen design by including safe targeting guides and short gRNAs.

Sept. 13, 2018, 1:55 p.m.