We narrowed to 529 results for: arl
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  TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m.... systems, developed and deposited at Addgene by Arlene Sharpe’s lab, allow for wide deployment of in vivo...here! Marty LaFleur is a Postdoctoral Fellow in Arlene Sharpe’s laboratory at Harvard Medical School and...
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  CRISPR Protocol for Genomic Deletions in Mammalian Cell Lines [Video]TypeBlog PostPublishedFeb. 18, 2015, 3:09 p.m....production of deletions by paired DSBs may be particularly useful. The first is the study of non-coding...genomic deletion efficiency mediated by clustered regularly interspaced palindromic repeats (CRISPR)/Cas9 ...
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  "What Makes a Good Mentor?" and 6 More FAQs About Science MentoringTypeBlog PostPublishedDec. 17, 2013, 2:47 p.m....researchers who joined a recent Twitter chat for Early Career Researchers. Many of the participants had...reach your goals Listens well and communicates clearly and openly Gives honest feedback without ever being...
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  Same Addgene, New Look - Why We Redesigned Our Homepage & MascotTypeBlog PostPublishedDec. 5, 2019, 1:49 p.m....is still important. It needs to express value, clearly communicate exactly what a site offers to their...communicate that sense of community, Blugene will regularly be paired with a variety of science-inspired friends...
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  New Norepinephrine Indicators: nLightG and nLightRTypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....alpha-1a adrenergic receptor; nLightG contains circularly permuted green fluorescent protein (GFP), and...green fluorescent proteins. nLightR did perform similarly to GRABNE, and having both colors allows for increased...
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  In Living Color: The Skinny on In Vivo Imaging ToolsTypeBlog PostPublishedMarch 27, 2014, 5:14 p.m.... spectral region in which mammalian tissues are nearly transparent. As Verkhusha explained it to me, the...tissues 20-fold greater than any fluorescent protein earlier described. “Until our proteins, there were lots...
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  Cellosaurus: A Cell Line Information DatabaseTypeBlog PostPublishedFeb. 21, 2023, 2:15 p.m....new resources with the scientific community – particularly ones that help address ongoing problems, like...growing cell line information database describing nearly 145,000 cell lines. Cellosaurus Cellosaurus seeks...
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  Year of the Camelid: Antibody StyleTypeBlog PostPublishedMay 9, 2024, 1:15 p.m.... to manipulate cellular functions, like in this early example we highlighted back in 2015, where sdAbs... So where do all these sdAbs come from? In the early years of sdAbs development, the process started ...
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  CASTing Off for New Shores in Human Genome EditingTypeBlog PostPublishedJune 10, 2025, 1:15 p.m....popular bacterial genome engineering tools, with early reports of 40%–60% efficient integration (Klompe... al., 2019). Type I-F CASTs were found to be particularly useful. But they were barely effective at editing...
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  Hot Plasmids and Viral Preps - July 2021TypeBlog PostPublishedJuly 27, 2021, 1:15 p.m....internal control and a SL-sensitive fluorophore (circularly permuted green fluorescent protein or cpGFP) ... New from the viral service By Jason Nasse We regularly add new viral aliquots from our plasmid collection...
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  Fostering communication in remote collaborationsTypeBlog PostPublishedApril 21, 2020, 1:14 p.m....experiments. Therefore, it’s crucial that everyone meets early on to set expectations for the output that will ... which parts of the project, and, as mentioned earlier, how frequently the entire group will meet. You...
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  Tips for Improving Your Next ManuscriptTypeBlog PostPublishedNov. 22, 2016, 3:30 p.m....consisted of graduate students, post-grads, and early career scientists. Over the course of the class,...welcoming us. Then it was time to work! Set up similarly to the online class, we met as a group for sessions...
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  Running for Rare Disease, Running for FOP, Running for AJTypeBlog PostPublishedSept. 15, 2016, 2:30 p.m.... in Cambridge, MA. When I joined Sanofi/Genzyme nearly three years ago I decided it was time to get in...analytical development. His areas of expertise include early stage drug discovery and pre-clinical development...
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  Addgene at the ASM Microbe 2016 ConferenceTypeBlog PostPublishedJuly 7, 2016, 2:30 p.m....Labeling Plasmids from the Barbier lab). Bright and early on Saturday morning, John Doench’s opening presentation... to the scientific community. However, from the nearly 4000 posters presented at the conference, it looks...
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  Walking beside Academia and giving it a high five: My transition from graduate work to a position at AddgeneTypeBlog PostPublishedJuly 2, 2015, 8:37 p.m....have been unwise of me to join Addgene. This is particularly true in my position as an Outreach Scientist...pretty hefty bumps during my graduate training. I particularly think that too many young scientists are led...
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  Addgene @ Keystone: Thoughts on Precision Genome Engineering and SynbioTypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....reagents so freely with the academic community has clearly made a huge impact on this field. Advances in ...answering biology customer service questions for nearly 7 years, and my colleagues and I have an internal...
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  10 Ways to Share Your Science!TypeBlog PostPublishedMay 13, 2014, 8:34 p.m....some science on the one you already have. Read my earlier post about Scientists on Twitter here. 4) If ...commitment, start your own blog. Plan to write regularly and, when you are ready, tell your friends or ...
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  What the HEK?TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....two areas of research where HEK cells may be particularly useful due to their intrinsic properties. ...by mycoplasma and bacteria, but HEKs are also particularly vulnerable to viral infection. Yes, your cells...
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  Safe Port in a Storm...How Addgene is Weathering the PandemicTypeBlog PostPublishedSept. 17, 2020, 1:15 p.m....expected to be in this here and now. Our closure was earlier than most companies. Since the start of this crisis... to make most of our payroll for the spring and early summer months. We are also extremely grateful to...
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  Single-cell tracking of lineage and identity with CellTagTypeBlog PostPublishedMay 9, 2019, 1:15 p.m....time point, making it difficult to investigate how early expression patterns influence reprogramming outcomes...However, distinct transcriptional states were seen as early as day 6. All together these results suggest that...