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Showing: 41 - 60 of 112 results

  1. CRISPR Plasmids - Base Edit

    Type
    Collection
    ...nickase or catalytically inactive “dead” Cas9 (dCas9) to a cytidine deaminase like APOBEC. Base editors...creating an A to G (or T to C) change. Adenine DNA deaminases do not exist in nature, but have been created... of the Escherichia coli TadA, a tRNA adenine deaminase. Like cytosine base editors, the evolved TadA ...modifying the linker region between Cas variant and deaminase to adjust the editing window, or adding fusions... Cas9 is fused to activation-induced cytidine deaminase (AID). Want more information on the wide variety...

  2. Genomic Deletions in Mammalian Cell Lines

    Type
    Collection
    ... and H 2 O to final volume of 50 μl. This reaction may be scaled down to a smaller final volume if necessary...video, you can find the protocol section from the original publication below. You can also download the full-text...., sgRNA-B in Table 4 ) and do not add C to the final position of the reverse complement oligo. In this...Buffer, 6.5 μl ddH 2 O, and 0.5 μl T4 Polynucleotide Kinase (PNK) (10,000 U/ml). NOTE: Phosphorylated oligos...contains 50 μl cell culture media per well for a final volume of 150 μl. This facilitates screening by ...research. Gehl J. Acta Physiologica Scandinavica . 2003. PubMed . Purifying plasmid DNA from ...

  3. Control AAV Preps

    Type
    Collection
    ... 5, rg*, PHP.eB Gradinaru 104061 CAG-NLS-GFP CAG NLS-GFP Constitutive PHPeB Gradinaru 105530 pAAV.CMV....NLS-mRuby2 Constitutive 1, 2, 8, 9, rg*, PHP.eB Gradinaru 100896 pAAV.GFA104.PI.eGFP.WPRE.bGH GFA104 EGFP...hSyn eYFP Constitutive 2, 5, 8, 9, rg*, PHP.eB Gradinaru 131000 pAAV-CaMKIIa-mScarlet CamKIIa mScarlet ...pAAV-CAG-DIO-EYFP CAG EYFP Cre dependent PHP.V1 Gradinaru 83895 pAAV-hDlx-Flex-GFP-Fishell_6 Dlx GFP Cre...pAAV-CAG-fDIO-mNeonGreen CAG mNeonGreen Flp dependent 1 Gradinaru 137131 pAAV-Ef1a-Coff/Fon-BFP EF1a BFP Flp dependent...

  4. MAPK Plasmids

    Type
    Collection
    ...Human Kinase ORFs Genome Engineering Guide Empty-Backbone Resource Mitogen-activated protein kinases (MAPKs...extracellular signal-regulated kinases (ERKs), are serine/threonine-specific protein kinases. They regulate diverse...Browse mitogen-activated protein kinase (MAPK) plasmids. Plasmid...

  5. Plant Plasmids and Resources

    Type
    Collection
    ...innovation for improvement of sustainable agriculture and conservation. Medicinal Plant Genomics Resource (...other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome...in a new window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible...Transcriptomic and metabolomic resources for 14 key medicinal plants for the advancement of drug production ...

  6. CRISPR Plasmids - Mammalian Expression

    Type
    Collection
    ... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can...MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window...the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...Marker PI Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP...

  7. Fujii Lab CRISPR Plasmids

    Type
    Collection
    ...Protocols gRNA Design Tools CRISPR Blog Posts Originally developed in 2009 (Hoshino & Fujii), the locus-specific...the CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for...chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. Fujita T, Yuno M, ...non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing. Fujita T, Yuno ...molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins.Fujita T, Fujii...

  8. CRISPR References and Information

    Type
    Collection
    ...sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis...Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed to identify zinc finger nuclease sites...and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative...pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid summary... , pAC1 , pAC147 , pAC148 , pAC149 ; gateway destination: pAC90 PDF 1.0 MB Katic Nematode: Cas9 and gRNA...

  9. Neurodegeneration Plasmid Collection

    Type
    Collection
    ...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...17800 pM-ErbB4-delta-kinase ERBB4 GAL4-BD ALS Marius Sudol 17801 pM-ErbB4-delta-kinase-PY1 mutant ERBB4 GAL4... GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD ALS Marius Sudol 17803 ... CMV Parkinson's Mark Cookson 25071 2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2...ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1...ERBB4 CMV ALS Yardena Samuels 29544 pCDF1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29591 426Gal-FUS...type IE Arthur Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1...

  10. NETRF

    Type
    Collection
    ... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

  11. Jaenisch Lab CRISPR Plasmids

    Type
    Collection
    ...Table 4. Gateway Destination Vector ID Plasmid 48222 pAC90-pmax-DEST : Gateway Destination vector derived...48218~48221] and can be transferred to Gateway destination vectors (e.g., pmax-DEST [48222] and (Link opens...opens in a new window) Lifetechnologies Gateway Destination Vectors ) to adapt to different systems by LR...

  12. Synthetic Biology - Overview

    Type
    Collection
    ... Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors...synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains...Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource ...

  13. Lentiviral Prep Service

    Type
    Collection
    ...18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries 92385 Human CRISPR inhibition...Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal...

  14. Genetic Code Expansion

    Type
    Collection
    ...Mb-Fluoro-Phe B5 Pyl Fluoro-Phe B5 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...Mb-Fluoro-Phe D6 Pyl Fluoro-Phe D6 M. barkeri fluorinated phenylalanine derivatives Mammalian TAG Ryan ...sites changes to UAG, RF1 function removed, ClpS inactivated, with Ubiquitin-UAG-sfGFP reporter George Church...codon, and tyrU gene replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses...tyrTV and tyrU genes replaced by tRNA variant inactivated by the truncation of the anticodon loop). Expresses...

  15. Fluorescent Protein Guide: Biosensors

    Type
    Collection
    ...factors and the sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo , ...Zhou Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids...biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. eLife. 2018 May 29;7....charges in live cells. Nat Biotechnol. 2017 Mar 13. Katharina Gaus Endo/Exocytosis Superecliptic pHluorin fused...-pHluorin Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal ...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy...

  16. Tags and Other Markers

    Type
    Collection
    ...Find recombinant antibodies targeting epitope tags and common fusion proteins....common cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

  17. Neuroscience

    Type
    Collection
    ...Find recombinant antibodies targeting neuroscience-related proteins....in neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

  18. Depositor Collections

    Type
    Collection
    ...Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection CRISPR CRISPR-on: an RNA-guided... Simpson Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project ... Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral...

  19. Fluorescent Protein Guide: In Vivo Imaging

    Type
    Collection
    ...Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600... in living animals. Further development of the original iRFP has resulted in a split fluorescence complementation...

  20. All Antibodies

    Type
    Collection
    ...Browse Addgene's collection of recombinant monoclonal antibodies....Addgene distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-...Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype Reactivity...
Showing: 41 - 60 of 112 results