...feedback loop for amplified tTA expression. 1 Gradinaru 99121 pAAV-ihSyn1-DIO-tTA Cre-dependent and inducible...feedback loop for amplified tTA expression. 1 Gradinaru 117383 TRE-DIO-eYFP Cre-dependent and Tetracycline-inducible...Cre-dependent, Tet-inducible expression of EYFP 1 Gradinaru Tools for Affinity Purification These AAV encode...tdTomato and synaptophysin-EGFP for labeling of axon terminals. 1 Zeng 71760 pAAV hSyn FLEx mGFP-2A-Synaptophysin-mRuby... and synaptophysin-mRuby for labeling of axon terminals. 1 Luo Tools for Cell Ablation These AAV encode...

...innovation for improvement of sustainable agriculture and conservation. Medicinal Plant Genomics Resource (...other monocots - Jorge Dubcovsky. Engineered biocontainable RNA virus vectors for non-transgenic genome...in a new window) - The initiative promotes interdisciplinary exchange, open technologies, and responsible...Transcriptomic and metabolomic resources for 14 key medicinal plants for the advancement of drug production ...

... Catalytically dead dCas9 fused to a cytidine deaminase protein becomes a specific base editor that can...MS2 hairpin-tagged gRNA to recruit a cytidine deaminase and induce somatic hypermutation in a 100 bp window...the catalytic domain of ADAR2(E488Q) adenosine deaminase to catalytically dead Cas13b creates a programmable...Selectable Marker PI Publication Purify A catalytically inactive Cas9 (dCas9) can be used to purify a region of...Marker PI Publication Visualize A catalytically inactive Cas9 (dCas9) fused to a fluorescent protein (FP...

...Protocols gRNA Design Tools CRISPR Blog Posts Originally developed in 2009 (Hoshino & Fujii), the locus-specific...the CRISPR system consisting of a catalytically inactive form of Cas9 (dCas9) plus guide RNA (gRNA) for...chromatin by in vitro enChIP technology using recombinant CRISPR ribonucleoproteins. Fujita T, Yuno M, ...non-coding RNAs associated with telomeres using a combination of enChIP and RNA sequencing. Fujita T, Yuno ...molecular interactions by the iChIP system using recombinant exogenous DNA-binding proteins.Fujita T, Fujii...

...sites by off-target quality. Validated for gene inactivation, NHEJ, and HDR. Reference genomes include Arabidopsis...Proof-of-concept performed in zebrafish. ZiFiT Targeter Originally developed to identify zinc finger nuclease sites...and selection for Cas9-triggered homologous recombination Cas9-sgRNA target construct ; pMA122 (negative...pharyngeal co-injection marker); pDD104 (Cre recombinase) PDF 102.5 KB Jaenisch CRISPR plasmid summary... , pAC1 , pAC147 , pAC148 , pAC149 ; gateway destination: pAC90 PDF 1.0 MB Katic Nematode: Cas9 and gRNA...

...Find unique recombinant antibodies targeting families of cell surface and secreted proteins from a collaboration...Innovation (IPI) Collection! Here, you’ll find unique recombinant antibodies targeting families of cell surface...window) and an optimized sorting process. This combination allows IPI to generate antibodies to highly conserved...Figure courtesy of IPI Available Antibodies ID Recombinant Antibody Description Isotype Recommended Applications...

... Cancer Institute Finding the Causes of Small Intestinal Neuroendocrine Tumors Ramesh Shivdasani Dana-...Dana-Farber Cancer Institute Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Qiao Zhou...Zhou Harvard University Epigenetic Regulators of Intestinal Endocrine Cells and Carcinoid Tumors Bradley ...Identifying Altered Epigenetic States and Drivers in Intestinal Carcinoid and Pancreatic Neuroendocrine Tumors...

...developing mouse retina. To aide in their studies of the regulatory networks involved in retinal development...plasmids to study the regulatory networks involved in retinal development. Depositor...967.2 KB Plasmid and Sequence Derivative Sources Original pX458 backbone ( Addgene #48138 ) dCas9 + KRAB-MeCP2...

...Table 4. Gateway Destination Vector ID Plasmid 48222 pAC90-pmax-DEST : Gateway Destination vector derived...48218~48221] and can be transferred to Gateway destination vectors (e.g., pmax-DEST [48222] and (Link opens...opens in a new window) Lifetechnologies Gateway Destination Vectors ) to adapt to different systems by LR...

... Kit Rinehart Recombinant Phosphoprotein Kit S. cerevisiae Advanced Gateway Destination Vectors TAL Effectors...synthetic regulatory elements, including promoters, terminators, repressors, activators, and more. Also contains...Mukhopadhyay Lab Efflux Pumps Voigt Lab Repressors , Terminators , Light Signaling , Sigma Factors , Resource ...

...CMV ALS Trina Schroer 51221 CMV-CC2 DCTN1 CMV ALS Trina Schroer 51412 CMV-p150 DCTN1 CMV ALS Trina Schroer...17800 pM-ErbB4-delta-kinase ERBB4 GAL4-BD ALS Marius Sudol 17801 pM-ErbB4-delta-kinase-PY1 mutant ERBB4 GAL4... GAL4-BD ALS Marius Sudol 17802 pM-ErbB4-delta-kinase-PY3 mutant ERBB4 GAL4-BD ALS Marius Sudol 17803 ... CMV Parkinson's Mark Cookson 25071 2XMyc-LRRK2-Kinase LRRK2 Myc CMV Parkinson's Mark Cookson 25072 2XMyc-LRRK2...ERBB4 CMV ALS Yardena Samuels 29533 pcDNA3.1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29534 pcDNA3.1...ERBB4 CMV ALS Yardena Samuels 29544 pCDF1-ERBB4 (kinase dead) ERBB4 CMV ALS Yardena Samuels 29591 426Gal-FUS...type IE Arthur Riggs 37057 GFP-SMN SMN1 GFP CMV Spinal muscular atrophy Greg Matera 38005 pET-21a(+)/MBP-His-PINK1...

...18,885 human genes Puromycin Doench , Root Human inactivation pooled libraries 92385 Human CRISPR inhibition...Celltag library contains 19973 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 4934 barcodes to combinatorially index cells for single-cell analysis of clonal... Celltag library contains 5737 barcodes to combinatorially index cells for single-cell analysis of clonal...

...Find recombinant antibodies targeting epitope tags and common fusion proteins....common cellular proteins. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

...Find recombinant antibodies targeting neuroscience-related proteins....in neuroscience research. These ready-to-use recombinant monoclonal antibodies undergo application-specific...and their use, visit our Antibody Guide . ID Recombinant Antibody Description Source Species Isotype Reactivity...

...factors and the sensor behavior may differ from the original characterization (e.g. in vitro vs. in vivo , ...Zhou Calcium Twitch calcium indicator, based on C-terminal domain of troponin C Griesbeck Lab Twitch Plasmids...biosensors for mapping cellular concentrations of nicotinamide adenine dinucleotides. Elife. 2018 May 29;7....charges in live cells. Nat Biotechnol. 2017 Mar 13. Katharina Gaus Endo/Exocytosis Superecliptic pHluorin fused...-pHluorin Clathrin-mediated endocytosis is the dominant mechanism of vesicle retrieval at hippocampal ...expression of pH sensitive mt-mKeima) The ubiquitin kinase PINK1 recruits autophagy receptors to induce mitophagy...

...Pooled Libraries Antibodies NeuroMab/Trimmer Lab Recombinant mAb Plasmid Collection CRISPR Casilio CRISPR/... Simpson Reagents for improved expression of recombinant phosphoproteins - Rinehart FreeGenes Project ... Repressor Plasmids Sigma (σ) Factor Plasmids Terminator Plasmids Viral Vectors pLEG/pREG Modular Viral...

...Emission Brightness Find Plasmids mCardinal 604/659 18 All mCardinal plasmids from article mNeptune 600... in living animals. Further development of the original iRFP has resulted in a split fluorescence complementation...

...you have the right to use the original plasmid. Distribution of the original plasmid is important because...which is a sample of the purified DNA from the original plasmid that was used to make the virus. In order...

...Browse Addgene's collection of recombinant monoclonal antibodies....Addgene distributes ready-to-use recombinant antibodies. These monoclonal antibodies undergo application-...Antibody Suggestion Form . Browse All Antibodies ID Recombinant Antibody Description Source Species Isotype Reactivity...

...genes in E. coli vectors with various N- and C-terminal tags for protein expression and/or purification...genes in yeast expression vectors with various C-terminal tags for protein expression and/or purification...mammalian expression vectors with various N- and C-terminal tags for protein expression and/or purification...