Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pEF-BOS
Information
- Plasmid Type
- Mammalian Expression
- Cloning Method
- Unknown
- Size
- 5800
- Bacterial Resistance
- Ampicillin
- Notes
- Mizushima and Nagata, Nucleic Acids Research, 18(17)5322. SV40 ori (311 bp), human EF-1alpha promoter (1.2 kb), stuffer fragment from CDM8 vector (450 bp, cDNA to be expressed can be inserted by removing the stuffer using BstXI or XbaI), poly-A from human G-CSF (700 bp Eco81I-EcoRI fragment), into HindIII-EcoRI site of pUC119. More information: Mizushima and Nagata, Nucleic Acids Research, 18(17)5322.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Nonviral