Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pGCAT-C
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 4552
- Notes
- Restriction digests of the clone give the following sizes (kb): PvuII--3.0, 1.5; HincII--3.0, 1.5; EcoRI--4.25, 0.3. (ATCC staff) The polylinker is in opposite orientation in pGCAT-A (ATCC 37640) and pGCAT-C (ATCC 37641). The sequence at the junction of the multiple cloning site and CAT is GAATTCGAGC and at the junction of SV40 and SP6 is GGATCAGCTT. (personal communication) BamHI/HindIII fragment of pSB1 (CAT) was cloned into the HindIII site of pGEM-4. 2 hrs after ligation began the remaining BamHI and HindIII sites were filled in. Contains SV40 polyadenylation site and donor and acceptor sites of SV40 small-t intron. A plasmid shuttle vector for investigating promoter and/or enhancer elements. Has a polylinker upstream of reporter CAT gene, SP6 and T7 promoters for in vitro RNA synthesis and low basal CAT expression in eukaryotic cells. Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, vertebrate cells, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 37641
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified