Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
This vector is not available from Addgene.
Plasmid: pMTH
Information
- Plasmid Type
- Mammalian Expression
- Promoter
- mouse metallothionein 1 promoter
- Cloning Method
- Unknown
- Size
- 11500
- Bacterial Resistance
- Ampicillin
- Selectable Marker
- Neomycin
- Notes
- Information retrieved from reference: McClinton et al, Mol Cell Biol 10, p 705-710. Plasmid contains (i) the mouse metallothionein 1 promoter, (ii) a genomic segment including the 3' end of exon 2, intron 2, exon 3, and 3'-flanking sequences of the human b-globin gene, and (iii) an SV40-Neo selectable marker was obtained from Jeffery Holt (Vanderbilt University). This plasmid was constructed by cloning the EcoRI-BglII promoter region into pUC-9 and then isolating this sequence as an EcoRI-HindIII fragment, which was used to replace the EcoRI-HindIII mouse mammory tumor virus sequence in the pHfos vector.
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Nonviral