Vector Database
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Vector database is a digital-only collection of vector backbone information compiled by Addgene from third party sources.
This vector is NOT available from Addgene and the database is no longer actively maintained.
Plasmid: pSE640
Information
- Source/Vendor
- ATCC
- Plasmid Type
- Unspecified
- Cloning Method
- Unknown
- Size
- 9000
- Notes
- YC-type shuttle enhancer-cloning vector using beta-galactosidase activity as the reporter. (personal communication) The order of the major features in this plasmid is: MCS - CYC - lacZ - pMB1 ori - ampR - URA3 - ARS1 - SUP11 - CEN4. (personal communication) The UAS has been deleted from the CYC1 promoter, so expression of a CYC1-lacZ fusion protein depends on UAS elements cloned into polylinker region. (personal communication) Restriction digests of the clone give the following sizes (kb): EcoRI--9.2; XhoI--9.2; SalI--9.2. (ATCC staff) Medium is 1227 LB plus ampicillin. Hosts: E.coli HB101, Saccharomyces cerevisiae, E.coli. (Information source: VectorDB ( http://seq.yeastgenome.org/vectordb ).)
- Catalog Number
- 77272
- Stable
- Unspecified
- Constitutive
- Unspecified
- Viral/Non-Viral
- Unspecified