pGEX4T3-Stx3-E78A/E83A
(Plasmid
#100272)
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PurposeExpresses GST-Stx3-E78A/E83A in bacteria.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 100272 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepGEX4T3
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Backbone manufacturerGE Lifesciences
- Backbone size w/o insert (bp) 4968
- Total vector size (bp) 5838
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameStx3-1-265-E78A/E83A
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SpeciesR. norvegicus (rat)
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Insert Size (bp)870
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MutationNo transmembrane domain; residues 4-264 present, changed both E78 and E83 to alanines
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Entrez GeneStx3 (a.k.a. Stx3a)
- Promoter Tac
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Tag
/ Fusion Protein
- GST (N terminal on backbone)
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer GGGCTGGCAAGCCACGTTTGGTG (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pGEX4T3-Stx3-E78A/E83A was a gift from Thomas Weimbs (Addgene plasmid # 100272 ; http://n2t.net/addgene:100272 ; RRID:Addgene_100272) -
For your References section:
The H(abc) domain of syntaxin 3 is a ubiquitin binding domain. Giovannone AJ, Reales E, Bhattaram P, Nackeeran S, Monahan AB, Syed R, Weimbs T. Sci Rep. 2020 Dec 7;10(1):21350. doi: 10.1038/s41598-020-78412-0. 10.1038/s41598-020-78412-0 PubMed 33288783
