Purpose(Empty Backbone) EMMA toolkit part-entry vector position 9
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100619||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSMART HC_Kan
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
It is used to standardize new parts for EMMA toolkit. New DNA fragments (parts) are cloned into this plasmid replacing the RFP gene through a BsaI Golden Gate reaction. The resulting plasmid will have the part flanked by BsmBI sites and the fusion sites corresponding to position 9 of EMMA platform.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCe1699 HC_Kan_RFP-p9 was a gift from Yizhi Cai (Addgene plasmid # 100619 ; http://n2t.net/addgene:100619 ; RRID:Addgene_100619)
For your References section:EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644