PurposeEMMA toolkit Assembly connector W-Z
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100664||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSMART HC_Kan
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEMMA toolkit Assembly connector W-Z
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
DNA element with no function. It is used as a bridge to fill in the gap between parts placed in position 22 of EMMA platform and the backbone
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCe2721 HC_Kan_conn_W-Z was a gift from Yizhi Cai (Addgene plasmid # 100664 ; http://n2t.net/addgene:100664 ; RRID:Addgene_100664)
For your References section:EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644