PurposeEMMA toolkit Assembly connector for second step assembly
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100671||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSMART HC_Kan
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameEMMA toolkit Assembly connector for second step assembly
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
DNA element with no function and internal BsaI site generating a TCCG overhang upon cleavage. It is used in the first step BsmBI-based Golden Gate along with HC_Kan_conn_BsaI-3_p1 to flank the vector with BsaI stes. Vectors built in the first BsmBI-based Golden Gate reaction will be flanked by BsaI sites that are used in a second step assembly using as entry vector HC_RFP-4 (the part-entry vector for position 4). See manuscript for futher information
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCe2730 HC_Kan_conn_BsaI-4_p25 was a gift from Yizhi Cai (Addgene plasmid # 100671 ; http://n2t.net/addgene:100671 ; RRID:Addgene_100671)
For your References section:EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644