Purposepart designed to occupy position 21 of EMMA. Functional category: Selection marker
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100691||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSMART HC_Kan
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Puromycin resistance gene ecnoding a puromycin N-acetyl-transferase.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCe2040 HC_Kan_PuroR_p21 was a gift from Yizhi Cai (Addgene plasmid # 100691 ; http://n2t.net/addgene:100691 ; RRID:Addgene_100691)
For your References section:EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644