Purposepart designed to occupy position 10 of EMMA. Functional category: Long Terminal Repeat
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||100703||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepSMART HC_Kan
Vector typeSynthetic Biology
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameLentiviral 3' LTR
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
- 3′ cloning site Unknown (unknown if destroyed)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
3'LTR for lentivial vectors. The 3'LTR included the�woodchuck hepatitis virus posttranscriptional regulatory element (WPRE) which stabilised the RNA, and the�self-inactivating 3' long terminal repeat (LTR) from HIV-1 (3' LTR (?U3))
IMPORT NOTES: Special character found in field(Comments)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:YCe3766 HC_Kan_3'-LTR_p10 was a gift from Yizhi Cai (Addgene plasmid # 100703 ; http://n2t.net/addgene:100703 ; RRID:Addgene_100703)
For your References section:EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors. Martella A, Matjusaitis M, Auxillos J, Pollard SM, Cai Y. ACS Synth Biol. 2017 Apr 24. doi: 10.1021/acssynbio.7b00016. 10.1021/acssynbio.7b00016 PubMed 28418644