|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||101715||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector backbonepx459 v2
- Total vector size (bp) 9175
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameSpCas9 VQR
MutationVQR (D1135V, R1335Q and T1337R)
/ Fusion Proteins
- 3xFLAG-NLS (N terminal on insert)
- NLS (C terminal on insert)
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:px459 VQR was a gift from Andrew Holland (Addgene plasmid # 101715 ; http://n2t.net/addgene:101715 ; RRID:Addgene_101715)