BL21ΔABCF
(Bacterial strain #102270)

• Purpose: ompA, lamB, ompC, ompF quadruple knockout strain for overproduction of outer membrane proteins
• Depositing Lab: Jack Leo
• Publication: Meuskens et al Front Cell Infect Microbiol. 2017 Nov 7;7:464. doi: 10.3389/fcimb.2017.00464. eCollection 2017.

Backbone

• Vector backbone: none

Growth in Bacteria

• Bacterial Resistance(s): Tetracycline, 10 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): BL21ΔABCF
• Growth instructions: Requires low salt (5 g/L NaCl or less) in LB with Tet. Strain is intrinsically tetracycline-resistant (10ug/mL). Bacteria may require 2 days for growth.
• Copy number: Unknown

Resource Information

• Supplemental Documents:
  • Genotypes and Primers
  • PCR results from Leo Lab
• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Genotype = ΔompA ΔompC ΔompF ΔlamB
Precursor strain = BL21Gold(DE3) [genotype F- ompT hsdS(rB– mB– ) dcm+ Tetr gal λ(DE3) endA Hte]

Supplemental document contains a list of genotypes and PCR primers used for verification of each knocked-out gene.

Due to the strain's growth defect and potential for contamination, Addgene recommends streaking for single colonies from the stab on low salt LB containing tetracycline and then screening colonies by PCR using the Leo Lab's recommended primers to confirm the strain.