|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||102995||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Total vector size (bp) 6505
Vector typeMammalian Expression, AAV
Growth in Bacteria
Growth Strain(s)NEB Stable
Growth instructionsAlthough Stable strains are highly recommended, regular strains (eg, DH5alpha) can be used for better yield.
Copy numberHigh Copy
SpeciesH. sapiens (human), Synthetic
- Cloning method Restriction Enzyme
- 5′ cloning site AscI (unknown if destroyed)
- 3′ cloning site NheI (unknown if destroyed)
- 5′ sequencing primer GTCGACTCCGGAATAACTTCGTA (Common Sequencing Primers)
Please visit https://www.biorxiv.org/content/10.1101/433953v1 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAV-synp-F-H2B-jRGECO1a was a gift from David Cox (Addgene plasmid # 102995 ; http://n2t.net/addgene:102995 ; RRID:Addgene_102995)
For your References section:Wide-area all-optical neurophysiology in acute brain slices. Farhi SL, Vicente VJ, Grama A, Yamagata M, Abdelfattah AS, Adam Y, Lou S, Kim JJ, Campbell RE, Cox DD, Cohen AE. bioRxiv 433953 10.1101/433953