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pcDNA3.1-R1ACR_447-EYFP
(Plasmid #103960)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 103960 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.1
  • Backbone manufacturer
    Invitrogen
  • Backbone size w/o insert (bp) 6217
  • Total vector size (bp) 7800
  • Modifications to backbone
    EYFP was cloned between NotI and XbaI sites
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Anion channelrhodopsin R1ACR_447
  • Species
    Rhodomonas sp.
  • Insert Size (bp)
    900
  • Mutation
    human codon optimized
  • GenBank ID
    KX879686
  • Promoter CMV
  • Tag / Fusion Protein
    • EYFP (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer T7
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.1-R1ACR_447-EYFP was a gift from John Spudich (Addgene plasmid # 103960 ; http://n2t.net/addgene:103960 ; RRID:Addgene_103960)
  • For your References section:

    The Expanding Family of Natural Anion Channelrhodopsins Reveals Large Variations in Kinetics, Conductance, and Spectral Sensitivity. Govorunova EG, Sineshchekov OA, Rodarte EM, Janz R, Morelle O, Melkonian M, Wong GK, Spudich JL. Sci Rep. 2017 Mar 3;7:43358. doi: 10.1038/srep43358. 10.1038/srep43358 PubMed 28256618