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pTECH-MmPylRS(IPYE)
(Plasmid #104073)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104073 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p15A
  • Backbone manufacturer
    Liu Lab
  • Total vector size (bp) 3943
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Plpp MmPylRS(IPYE); PproK pyl
  • Species
    Synthetic
  • Mutation
    Contains activating mutations V31I, T56P, H62Y, A100E (IPYE) in the MmPylRS gene
  • Promoter Plpp and PproK

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTECH-MmPylRS(IPYE) was a gift from David Liu (Addgene plasmid # 104073 ; http://n2t.net/addgene:104073 ; RRID:Addgene_104073)
  • For your References section:

    Continuous directed evolution of aminoacyl-tRNA synthetases. Bryson DI, Fan C, Guo LT, Miller C, Soll D, Liu DR. Nat Chem Biol. 2017 Oct 16. doi: 10.1038/nchembio.2474. 10.1038/nchembio.2474 PubMed 29035361