This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.
Addgene will be closed November 22nd & 23rd for the Thanksgiving holiday. Order processing and shipping may be delayed the week of Nov 19th - 23rd. If you have any questions, please contact us at [email protected]dgene.org.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pEF004-sgRNA-shuffle-in
(Plasmid #104440)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 104440 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pD000
  • Backbone manufacturer
    Lampropoulos et al., 2013
  • Total vector size (bp) 2934
  • Modifications to backbone
    A U6 promoter driven sgRNA-transRNA cassette is cloned into the backbone
  • Vector type
    Bacterial Expression, Plant Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    sgRNA-transRNA transcription by U6 promoter
  • Alt name
    gRNA-transRNA
  • Species
    A. thaliana (mustard weed)
  • Insert Size (bp)
    234
  • Promoter U6 promoter

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer M13 forward
  • 3′ sequencing primer M13 reverse
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEF004-sgRNA-shuffle-in was a gift from Detlef Weigel (Addgene plasmid # 104440 ; http://n2t.net/addgene:104440 ; RRID:Addgene_104440)
  • For your References section:

    An efficient CRISPR vector toolbox for engineering large deletions in Arabidopsis thaliana. Wu R, Lucke M, Jang YT, Zhu W, Symeonidi E, Wang C, Fitz J, Xi W, Schwab R, Weigel D. Plant Methods. 2018 Aug 2;14:65. doi: 10.1186/s13007-018-0330-7. eCollection 2018. 10.1186/s13007-018-0330-7 PubMed 30083222