PM PKA Reporter
(Plasmid #104551)

• Purpose: Expresses Lyn-mTurquoise2-VASP at the plasma membrane of mammalian cells
• Depositing Lab: David Lawrence
• Publication: O'Banion et al Cell Chem Biol. 2017 Oct 25. pii: S2451-9456(17)30355-0. doi: 10.1016/j.chembiol.2017.09.011.

Backbone

• Vector backbone: pEF1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4731
• Total vector size (bp): 5736
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Lyn-mTurquoise2-VASP 148 - 164
• Species: Synthetic
• Insert Size (bp): 855
• Promoter: EF1 alpha
• Tag / Fusion Protein:
  • mTurquoise 2, HA tag

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: AGATCTCGAGCTCAAGCTTC
• 3′ sequencing primer: GTAACCATTATAAGCTGCAA

Resource Information

• Supplemental Documents:
  • pEF1-Lyn-HA-mT-VASP.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Plasma membrane targeted PKA reporter. Lyn N-terminal myristoylation sequence fused to N-terminus of mTurquoise2 and VASP 148-164 fused to C terminus of fluorescent protein.