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pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus
(Plasmid #105127)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 105127 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Zebrafish fabp10a promoter:Xenopus pt-beta-catenin coding sequence, polyA
  • Species
    X. laevis (frog)
  • Insert Size (bp)
    5280
  • Entrez Gene
    fabp10a (a.k.a. L-B, L-F, L-FABP, Lb-F, Lb-FABP, Lfabp, Zf-FABP10, fabp, fabp10, lf, z-L-BABP, zgc:103719, zgc:92741)
  • Promoter fabp10a

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    Zebrafish cryaa promoter:Venus fluorescent protein coding sequence
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1516
  • Entrez Gene
    cryaa (a.k.a. wu:fc06h04, zgc:92036)
  • Promoter cryaa (crystallin)

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The Xenopus pt-beta-catenin sequence is derived from pCS2-XE49 (Randall Moon lab) The remainder of the sequence is derived from the pHD157 plasmid (Didier Stainier and Daniel Hesselson).
  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKE4-I-SceI-fabp10a:Xpt-β-cat, cryaa:Venus was a gift from Kimberley Evason & Didier Stainier (Addgene plasmid # 105127 ; http://n2t.net/addgene:105127 ; RRID:Addgene_105127)
  • For your References section:

    Identification of Chemical Inhibitors of beta-Catenin-Driven Liver Tumorigenesis in Zebrafish. Evason KJ, Francisco MT, Juric V, Balakrishnan S, Lopez Pazmino Mdel P, Gordan JD, Kakar S, Spitsbergen J, Goga A, Stainier DY. PLoS Genet. 2015 Jul 2;11(7):e1005305. doi: 10.1371/journal.pgen.1005305. eCollection 2015 Jul. PGENETICS-D-14-03252 [pii] PubMed 26134322